Western Blot (WB)
(Western Blot:Sample: Recombinant GLS, Human.)
Rabbit Glutaminase Antibody | anti-GLS antibody
Polyclonal Antibody to Glutaminase (GLS)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot:Sample: Recombinant GLS, Human.)
Western Blot (WB)
(Western Blot:Sample: Recombinant GLS, Human.)
Western Blot (WB)
(Western Blot:Lane 1: Rat Brain Tissue;Lane 2: Rat Kidney Tissue.)
Western Blot (WB)
(Western Blot:Lane 1: Rat Brain Tissue;Lane 2: Rat Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human GLS AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Cerebrum Tissue;Primary Ab: 20ug/ml Rabbit Anti-Human GLS AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2012]
Uniprot Description
glutaminase: Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate in the brain. Isoform 2 lacks catalytic activity. Belongs to the glutaminase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; EC 3.5.1.2; Energy Metabolism - nitrogen; Hydrolase; Mitochondrial; Other Amino Acids Metabolism - D-Glutamine and D-glutamate
Chromosomal Location of Human Ortholog: 2q32.2
Cellular Component: cytosol; mitochondrial matrix; mitochondrion
Molecular Function: glutaminase activity; protein binding
Biological Process: amino acid biosynthetic process; glutamate biosynthetic process; glutamate secretion; glutamine catabolic process; protein homotetramerization; regulation of respiratory gaseous exchange by neurological system process; suckling behavior; synaptic transmission
Research Articles on GLS
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Product Notes
The GLS gls (Catalog #AAA2026708) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Glutaminase (GLS) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Glutaminase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP). . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the GLS gls for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutaminase, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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