Rabbit Pulmonary surfatcant-associated protein B, SP-B ELISA Kit | SP-B elisa kit

Rabbit Pulmonary surfatcant-associated protein B, SP-B ELISA Kit

Cat. Num. AAA165804

• Gene Names: SFTPB; SP-B; PSP-B; SFTB3; SFTP3; SMDP1
• Reactivity: Rabbit
• Synonyms: Pulmonary surfatcant-associated protein B; SP-B; Rabbit Pulmonary surfatcant-associated protein B; SP-B ELISA Kit; SP-B elisa kit

• Reactivity: Rabbit
• Sequence Length: 393

• Samples: Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 2ng/L-700ng/L
• Sensitivity: 1.58ng/L
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SP-B elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rabbit Prostacycline (also known as PGI2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rabbit PGI2 antibody. PGI2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rabbit PGI2 Antibody is added and binds to PGI2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PGI2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rabbit PGI2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 288856299
• NCBI GeneID: 6439
• NCBI Accession #: NP_000533.3
• NCBI GenBank Nucleotide #: NM_000542.3
• UniProt Accession #: P07988; Q96R04
• Molecular Weight: 42,117 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein B
• NCBI Official Synonym Full Names: surfactant protein B
• NCBI Official Symbol: SFTPB
• NCBI Official Synonym Symbols: SP-B; PSP-B; SFTB3; SFTP3; SMDP1
• NCBI Protein Information: pulmonary surfactant-associated protein B
• UniProt Protein Name: Pulmonary surfactant-associated protein B
• UniProt Gene Name: SFTPB
• UniProt Synonym Gene Names: SFTP3; SP-B

NCBI Description

This gene encodes the pulmonary-associated surfactant protein B (SPB), an amphipathic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. The SPB enhances the rate of spreading and increases the stability of surfactant monolayers in vitro. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 1, also called pulmonary alveolar proteinosis due to surfactant protein B deficiency, and are associated with fatal respiratory distress in the neonatal period. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, Feb 2010]

Uniprot Description

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.