Rabbit CRTC2 / TORC2 Polyclonal Antibody | anti-CRTC2 antibody

Rabbit Polyclonal (IgG) to Human CRTC2 / TORC2

Cat. Num. AAA248187

• Gene Names: CRTC2; TORC2; TORC-2
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: CRTC2 / TORC2; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human CRTC2 / TORC2; Anti-CRTC2 / TORC2 Antibody (C-Terminus) IHC-plus; CRTC2; Transducer of CREB protein 2; TORC-2; TORC2; Human CRTC2; anti-CRTC2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Multiple isoforms of CRTC2 are known to exist. CRTC2 antibody is predicted to not cross-react with CRTC1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 693

• Applicable Applications for anti-CRTC2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (20 ug/ml), IHC-P (10 ug/ml), WB (0.5 - 2 ug/ml)
• Target Species: Human
• Immunogen Description: 16 amino acid peptide near the carboxy terminus of human CRTC2.
• Immunogen: CRTC2 / TORC2 antibody was raised against 16 amino acid peptide near the carboxy terminus of human CRTC2.
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CRTC2 / TORC2 antibody IHC staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-CRTC2 / TORC2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunofluorescence (IF)

(Immunofluorescence of CRTC2 in human small intestine tissue with CRTC2 antibody at 20 ug/ml.)

Western Blot (WB)

(Western blot analysis of CRTC2 in human small intestine tissue lysate with CRTC2 antibody at (A) 0.5 and (B) 1 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 32171215
• NCBI GeneID: 200186
• NCBI Accession #: NP_859066.1
• NCBI GenBank Nucleotide #: NM_181715.2
• UniProt Accession #: Q53ET0; Q6UUV8; Q7Z3X7; Q8N332
• Molecular Weight: 73,302 Da
• NCBI Official Full Name: CREB-regulated transcription coactivator 2
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 2
• NCBI Official Symbol: CRTC2
• NCBI Official Synonym Symbols: TORC2; TORC-2
• NCBI Protein Information: CREB-regulated transcription coactivator 2; transducer of regulated cAMP response element-binding protein (CREB) 2
• UniProt Protein Name: CREB-regulated transcription coactivator 2
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC2
• UniProt Synonym Gene Names: TORC2; TORC-2; Transducer of CREB protein 2
• UniProt Entry Name: CRTC2_HUMAN

NCBI Description

This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]

Uniprot Description

TORC2: the transducer of regulated CREB protein 2 (TORC2) is a cAMP responsive coactivator that, in concert with LKB1 and AMPK, controls glucose homeostasis in the liver. Under fasting conditions, cytoplasmic TORC2 is transported to the nucleus where it binds to CREB and stimulates gluconeogenisis. Re-feeding and the ensuing insulin response inhibits gluconeogenic gene expression by promoting the phosphorylation, cytoplasmic export and degradation of TORC2. TORC2 is phosphorylated by the kinase QIK which in turn is activated by phosphorylation at Ser358 by insulin-activated Akt2. Phosphorylated TORC2 is subsequently ubiquitinylated at Lys 628 and degraded by the 26S proteasome. TORC2 protein levels and activity are increased in diabetes owing to a block in TORC2 phosphorylation, implicating this pathway in the pathogenesis of diabetes. The CREB/TORC2 regulatory axis controls the normal pattern of germinal center (GC) B cell gene activation/repression that promotes B cell development and circumvents GC lymphomagenesis.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: protein binding; cAMP response element binding protein binding

Biological Process: viral reproduction; transcription, DNA-dependent; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; gluconeogenesis; protein homotetramerization