Western Blot (WB)
(Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted). Predicted band size:71kDa. Observed band size:71kDa.)
Rabbit TRIM25/EFP Monoclonal Antibody | anti-TRIM25 antibody
TRIM25/EFP Rabbit mAb
IHC: 1:20-1:100
ICC/IF: 1:50-1:200
FC/FACS: 1:20
IP: 1:20
Western Blot (WB)
(Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted). Predicted band size:71kDa. Observed band size:71kDa.)
Western Blot (WB)
(Western blot detection of TRIM25/EFP in K562,Rat Brain,C6,Hela cell lysates using TRIM25/EFP Rabbit mAb(1:1000 diluted). Predicted band size:71kDa. Observed band size:71kDa.)
Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
Uniprot Description
TRIM25: a ubiquitous protein that mediates estrogen action in various target organs. Contains one RING-type zinc finger and one SPRY domain.
Protein type: EC 6.3.2.n3; Ubiquitin conjugating system; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription factor; Ligase; EC 6.3.2.19
Chromosomal Location of Human Ortholog: 17q23.2
Cellular Component: nucleoplasm; cytoplasm; cytosol
Molecular Function: protein binding; acid-amino acid ligase activity; zinc ion binding; transcription factor activity
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; negative regulation of virion penetration into host cell; cytokine and chemokine mediated signaling pathway; protein ubiquitination; regulation of virion penetration into host cell; activation of NF-kappaB transcription factor; response to vitamin D; response to estrogen stimulus; innate immune response; positive regulation of transcription factor activity; negative regulation of interferon type I production; defense response to virus
Research Articles on TRIM25
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Product Notes
The TRIM25 trim25 (Catalog #AAA4753080) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM25/EFP Rabbit mAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM25/EFP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS), Immunoprecipitation (IP). WB: 1:1000-1:5000 IHC: 1:20-1:100 ICC/IF: 1:50-1:200 FC/FACS: 1:20 IP: 1:20. Researchers should empirically determine the suitability of the TRIM25 trim25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM25/EFP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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