Rabbit LPL ELISA Kit | LPL elisa kit

Rabbit LPL (Lipoprotein Lipase) ELISA Kit

Cat. Num. AAA2513977

• Gene Names: LPL; LIPD; HDLCQ11
• Reactivity: Rabbit
• Synonyms: LPL; Rabbit LPL (Lipoprotein Lipase) ELISA Kit; LPL elisa kit

• Reactivity: Rabbit
• Specificity: This kit recognizes natural and recombinant Rabbit LPL. No significant cross-reactivity or interference between Rabbit LPL and analogues was observed.
• Sequence Length: 475

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rabbit LPL concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPL elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LPL. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for LPL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LPL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of LPL. You can calculate the concentration of LPL in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 48146225
• NCBI GeneID: 4023
• UniProt Accession #: Q6IAV0; P06858
• Molecular Weight: 53,213 Da
• NCBI Official Full Name: LPL
• NCBI Official Synonym Full Names: lipoprotein lipase
• NCBI Official Symbol: LPL
• NCBI Official Synonym Symbols: LIPD; HDLCQ11
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: LPL protein
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Entry Name: Q6IAV0_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: EC 3.1.1.34; Phospholipase; Membrane protein, GPI anchor; Lipid Metabolism - glycerolipid

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; plasma membrane; extracellular region

Molecular Function: heparin binding; triacylglycerol lipase activity; protein binding; lipoprotein lipase activity; apolipoprotein binding; phospholipase activity; triglyceride binding; receptor binding

Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; lipoprotein metabolic process; triacylglycerol biosynthetic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process

Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined