Canine Lipoprteinlipase ELISA Kit | LPL elisa kit

Canine Lipoprteinlipase ELISA Kit

Cat. Num. AAA740146

• Gene Names: LPL; LIPD; HDLCQ11
• Reactivity: Canine
• Synonyms: Lipoprteinlipase; Canine Lipoprteinlipase ELISA Kit; LPL elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This LPL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine LPL. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LPL elisa kit

Principle of the Assay: LPL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LPL antibody and an LPL-HRP conjugate. The assay sample and buffer are incubated together with LPL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LPL concentration since LPL from samples and LPL-HRP conjugate compete for the anti-LPL antibody binding site. Since the number of sites is limited, as more sites are occupied by LPL from the sample, fewer sites are left to bind LPL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LPL concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LPL elisa kit

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 48146225
• NCBI GeneID: 4023
• UniProt Accession #: Q6IAV0; P06858; Q4JIZ7; Q53HW6; Q71UV2; Q8NEW3
• Molecular Weight: 53,213 Da
• NCBI Official Full Name: LPL
• NCBI Official Synonym Full Names: lipoprotein lipase
• NCBI Official Symbol: LPL
• NCBI Official Synonym Symbols: LIPD; HDLCQ11
• NCBI Protein Information: lipoprotein lipase
• UniProt Protein Name: LPL protein
• Protein Family: Lipoprotein
• UniProt Gene Name: LPL
• UniProt Entry Name: Q6IAV0_HUMAN

NCBI Description

LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]

Uniprot Description

LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Lipid Metabolism - glycerolipid; Membrane protein, GPI anchor; EC 3.1.1.34; Phospholipase

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; extracellular region; plasma membrane

Molecular Function: heparin binding; triacylglycerol lipase activity; protein binding; lipoprotein lipase activity; apolipoprotein binding; phospholipase activity; triglyceride binding; receptor binding

Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; lipoprotein metabolic process; triacylglycerol biosynthetic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process

Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined