Rabbit anti-Human GATA4 Polyclonal Antibody | anti-GATA4 antibody

Rabbit Polyclonal to Human GATA4

Cat. Num. AAA247146

• Gene Names: GATA4; TOF; ASD2; VSD1; TACHD
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified
• Synonyms: GATA4; Polyclonal Antibody; Rabbit Polyclonal to Human GATA4; Anti-GATA4 Antibody (aa330-380) IHC-plus; ASD2; GATA-binding factor 4; GATA-binding protein 4; GATA binding protein 4; Transcription factor GATA-4; VSD1; Human GATA4; anti-GATA4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Human GATA4
• Purity/Purification: Purified
• Form/Format: PBS, 0.05% BSA, 0.05% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 442

• Applicable Applications for anti-GATA4 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1 - 3 ug/ml)
• Target Species: Human
• Immunogen Description: A portion of amino acids 330-380 of human GATA-4 was used as the immunogen.
• Immunogen Type: Synthetic peptide
• Immunogen: GATA4 antibody was raised against a portion of amino acids 330-380 of human GATA-4 was used as the immunogen.
• Antigen Modification: aa330-380
• Preparation and Storage: Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-GATA4 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-GATA4 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.)

Western Blot (WB)

(Western blot of GATA-4 in human kidney lysate in the 1) absence and 2) presence of immunizing peptide using at 2 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.)

Related Product Information for anti-GATA4 antibody

GATA-4 is a zinc finger transcription factor that is essential for heart development. In mouse heart development, the first expression of GATA-4 is seen in coelomic epithelial cell of the primitive streak (7-7.5 days). During formation and bending of the heart tube, expression is found in endocardium, myocardium and embryonic structures containing precardiac mesoderm. By the onset of cardiac septation (9 days) expression is present in endocardium, endocardial cushion tissue and myocardium.

Product Categories/Family for anti-GATA4 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 33188461
• NCBI GeneID: 2626
• NCBI Accession #: NP_002043.2
• NCBI GenBank Nucleotide #: NM_002052.3
• UniProt Accession #: P43694; Q3MJ45; Q5IFM8; B7ZKX0; B7ZKZ4
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: transcription factor GATA-4
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: TOF; ASD2; VSD1; TACHD
• NCBI Protein Information: transcription factor GATA-4; GATA-binding factor 4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiac septal defects. [provided by RefSeq, Jul 2008]

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Motility/polarity/chemotaxis; Transcription factor

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; transcription activator binding; DNA binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: cardiac muscle hypertrophy; transcription from RNA polymerase II promoter; negative regulation of autophagy; positive regulation of transcription, DNA-dependent; embryonic heart tube anterior/posterior pattern formation; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; BMP signaling pathway; cell-cell signaling; regulation of transcription, DNA-dependent; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; response to mechanical stimulus; endoderm formation; response to estrogen stimulus; positive regulation of cardioblast differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; endoderm development

Disease: Ventricular Septal Defect 1; Tetralogy Of Fallot; Atrial Septal Defect 2; Atrioventricular Septal Defect 4; Testicular Anomalies With Or Without Congenital Heart Disease