Rabbit Cholesteryl Ester Transfer Protein (CETP) ELISA Kit | CETP elisa kit

Rabbit Cholesteryl Ester Transfer Protein (CETP) ELISA Kit

Cat. Num. AAA2019427

• Gene Names: CETP; BPIFF; HDLCQ10
• Reactivity: Rabbit
• Synonyms: Cholesteryl Ester Transfer Protein (CETP); Rabbit Cholesteryl Ester Transfer Protein (CETP) ELISA Kit; LTP; Lipid Transfer Protein; Plasma Lipid Transfer Protein; Cholesterylester Transfer Protein; CETP elisa kit

• Reactivity: Rabbit
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholesteryl Ester Transfer Protein (CETP).; No significant cross-reactivity or interference between Cholesteryl Ester Transfer Protein (CETP) and analogues was observed.
• Sequence Length: 425

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 46.88-3000pg/mL
• Sensitivity: < 20.53pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesteryl Ester Transfer Protein (CETP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CETP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesteryl Ester Transfer Protein (CETP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesteryl Ester Transfer Protein (CETP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesteryl Ester Transfer Protein (CETP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesteryl Ester Transfer Protein (CETP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CETP elisa kit

Metabolic pathway; Hepatology

NCBI and Uniprot Product Information

• NCBI GI #: 180271
• NCBI GeneID: 1071
• UniProt Accession #: P11597; Q13987; Q13988; Q53YZ1
• Molecular Weight: 47,787 Da
• NCBI Official Full Name: cholesteryl ester transfer protein, partial
• NCBI Official Synonym Full Names: cholesteryl ester transfer protein, plasma
• NCBI Official Symbol: CETP
• NCBI Official Synonym Symbols: BPIFF; HDLCQ10
• NCBI Protein Information: cholesteryl ester transfer protein
• UniProt Protein Name: Cholesteryl ester transfer protein
• Protein Family: Cholesteryl ester transfer protein
• UniProt Gene Name: CETP
• UniProt Entry Name: CETP_HUMAN

NCBI Description

The protein encoded by this gene is found in plasma, where it is involved in the transfer of cholesteryl ester from high density lipoprotein (HDL) to other lipoproteins. Defects in this gene are a cause of hyperalphalipoproteinemia 1 (HALP1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

CETP: Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol. Defects in CETP are the cause of hyperalphalipoproteinemia type 1 (HALP1). Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Lipid-binding; Vesicle

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: lipid transporter activity; phospholipid transporter activity; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding; triglyceride binding

Biological Process: receptor-mediated endocytosis; cholesterol metabolic process; cholesterol transport; lipid homeostasis; lipoprotein metabolic process; lipid transport; phosphatidylcholine metabolic process; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; phospholipid transport; triacylglycerol transport; transmembrane transport; phospholipid homeostasis

Disease: Hyperalphalipoproteinemia 1