Human Cholesteryl ester transfer protein ELISA Kit | CETP elisa kit

Human Cholesteryl ester transfer protein ELISA Kit

Cat. Num. AAA765783

• Gene Names: CETP; BPIFF; HDLCQ10
• Reactivity: Human
• Synonyms: Cholesteryl ester transfer protein; Human Cholesteryl ester transfer protein ELISA Kit; CETP/BPIFF/HDLCQ10/LTP/Plasma Lipid Transfer Protein/Lipid transfer protein I; CETP elisa kit

• Reactivity: Human
• Sequence Length: 493

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CETP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CETP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CETP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CETP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human CETP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 180260
• NCBI GeneID: 1071
• Molecular Weight: 47,787 Da
• NCBI Official Full Name: cholesteryl ester transfer protein
• NCBI Official Synonym Full Names: cholesteryl ester transfer protein, plasma
• NCBI Official Symbol: CETP
• NCBI Official Synonym Symbols: BPIFF; HDLCQ10
• NCBI Protein Information: cholesteryl ester transfer protein
• UniProt Protein Name: Cholesteryl ester transfer protein
• Protein Family: Cholesteryl ester transfer protein
• UniProt Gene Name: CETP
• UniProt Entry Name: CETP_HUMAN

NCBI Description

The protein encoded by this gene is found in plasma, where it is involved in the transfer of cholesteryl ester from high density lipoprotein (HDL) to other lipoproteins. Defects in this gene are a cause of hyperalphalipoproteinemia 1 (HALP1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Uniprot Description

CETP: Involved in the transfer of insoluble cholesteryl esters in the reverse transport of cholesterol. Defects in CETP are the cause of hyperalphalipoproteinemia type 1 (HALP1). Affected individuals show high levels of alpha-lipoprotein (high density lipoprotein/HDL). Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted; Vesicle; Lipid-binding

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: extracellular region; extracellular space; vesicle

Molecular Function: cholesterol binding; cholesterol transporter activity; lipid binding; lipid transporter activity; phosphatidylcholine binding; phospholipid transporter activity; triglyceride binding

Biological Process: cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; lipid homeostasis; lipid transport; lipoprotein metabolic process; phosphatidylcholine metabolic process; phospholipid homeostasis; phospholipid transport; receptor-mediated endocytosis; reverse cholesterol transport; triacylglycerol metabolic process; triacylglycerol transport

Disease: Hyperalphalipoproteinemia 1