Western Blot (WB)
(Western Blot: Sample: Human Jurkat Cells.)
Rabbit anti-Human Cyclin B Antibody | anti-CCNB antibody
Cyclin B (CCNB) Polyclonal Antibody
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Human Jurkat Cells.)
Western Blot (WB)
(Western Blot: Sample: Human Jurkat Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CCNB, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant CCNB, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Human Tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CCNB knockout Jurkat cell lysate; Predicted MW: 48,44kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human CCNB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: CCNB knockout Jurkat cell lysate; Predicted MW: 48,44kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human CCNB Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). The encoded protein is necessary for proper control of the G2/M transition phase of the cell cycle. [provided by RefSeq, Aug 2017]
Uniprot Description
CCNB1: a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Complexes with cdc2 to form the maturation-promoting factor (MPF). Two alternatively spliced isoforms have been found, a constitutively expressed form and a cell cycle-regulated form that is expressed predominantly during G2/M phase.
Protein type: Activator; Cell cycle regulation
Chromosomal Location of Human Ortholog: 5q13.2
Cellular Component: centrosome; condensed nuclear chromosome outer kinetochore; cytoplasm; cytosol; membrane; mitochondrial matrix; nucleoplasm; nucleus; spindle pole
Molecular Function: cyclin-dependent protein kinase activity; histone kinase activity; patched binding; protein binding; protein kinase binding; ubiquitin-like protein ligase binding
Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; cellular response to fatty acid; cellular response to hypoxia; cellular response to iron(III) ion; cellular response to organic cyclic compound; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; G2/M transition of mitotic cell cycle; gut development; histone H3-S10 phosphorylation involved in chromosome condensation; in utero embryonic development; mitotic metaphase plate congression; mitotic nuclear envelope disassembly; mitotic spindle organization and biogenesis; negative regulation of gene expression; negative regulation of protein phosphorylation; negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle; oocyte maturation; positive regulation of attachment of spindle microtubules to kinetochore; positive regulation of cardiac muscle cell proliferation; positive regulation of cyclin-dependent protein serine/threonine kinase activity involved in G2/M transition of mitotic cell cycle; positive regulation of fibroblast proliferation; positive regulation of histone phosphorylation; positive regulation of mitochondrial ATP synthesis coupled electron transport; positive regulation of mitotic cell cycle; positive regulation of mRNA 3'-end processing; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein complex assembly; protein ubiquitination involved in ubiquitin-dependent protein catabolic process; regulation of cell cycle; regulation of mitotic cell cycle spindle assembly checkpoint; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; response to DDT; response to drug; response to mechanical stimulus; spermatogenesis; tissue regeneration; transcription initiation from RNA polymerase II promoter; ventricular cardiac muscle cell development
Research Articles on CCNB
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Product Notes
The CCNB ccnb1 (Catalog #AAA2032449) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Cyclin B (CCNB) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Cyclin B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CCNB ccnb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cyclin B, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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