Rabbit anti-Human SOX3 Polyclonal Antibody | anti-SOX3 antibody

SOX3, ID (SOX3, Transcription factor SOX-3)

Cat. Num. AAA6005110

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: SOX3; Polyclonal Antibody; ID (SOX3; Transcription factor SOX-3); Anti -SOX3; anti-SOX3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-SOX3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: SOX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 173-204 amino acids from the Central region of human SOX3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SOX3 Antibody (Center) western blot analysis in MDA-MB453,HL-60 cell line lysates (35ug/lane).This demonstrates the SOX3 antibody detected the SOX3 protein (arrow).)

Related Product Information for anti-SOX3 antibody

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency.

Product Categories/Family for anti-SOX3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 530020
• UniProt Accession #: P41225; P35714; Q5JWI3; Q9NP49
• Molecular Weight: 45,210 Da
• NCBI Official Full Name: SOX3
• UniProt Protein Name: Transcription factor SOX-3
• Protein Family: Transcription factor
• UniProt Gene Name: SOX3
• UniProt Entry Name: SOX3_HUMAN

NCBI Description

This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. Mutations in this gene have been associated with X-linked mental retardation with growth hormone deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

SOX3: Transcription factor required during the formation of the hypothalamo-pituitary axis. May function as a switch in neuronal development. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Required also within the pharyngeal epithelia for craniofacial morphogenesis. Controls a genetic switch in male development. Is necessary for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Defects in SOX3 are a cause of panhypopituitarism X- linked (PHPX). Affected individuals have absent infundibulum, anterior pituitary hypoplasia, and ectopic posterior pituitary. Defects in SOX3 are the cause of mental retardation X- linked with isolated growth hormone deficiency (MRXGH). Defects in SOX3 are the cause of 46,XX sex reversal type 3 (SRXX3). A condition in which male gonads develop in a genetic female (female to male sex reversal). Copy number variations (CNV) encompassing or in close proximity to SOX3 are responsible for XX male reversal. These variations include two duplications of approximately 123 kb and 85 kb, the former of which spans the entire SOX3 gene; a 343 kb deletion immediately upstream of SOX3 that is probably responsible of altered regulation (and not increased dosage) of SOX3; a large (approximately 6 Mb) duplication that encompasses SOX3 and at least 18 additional distally located genes. Its proximal breakpoint falls within the SOX3 regulatory region. This large rearrangement has been found in a patient with XX male reversal and a complex phenotype that also includes a scrotal hypoplasia, microcephaly, developmental delay, and growth retardation.

Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: Xq27.1

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: sex determination; organ morphogenesis; spermatid differentiation; negative regulation of neuron differentiation; hypothalamus development; sensory organ development; central nervous system development; transcription, DNA-dependent; pituitary gland development; neuron development; negative regulation of transcription from RNA polymerase II promoter; Sertoli cell development

Disease: Panhypopituitarism, X-linked; Mental Retardation, X-linked, With Panhypopituitarism