Testing Data
Rabbit Rho Kinase/ROCKII (Paired T249) Antibody | anti-Rho Kinase/ROCKII (Paired T249) antibody
Rabbit anti ROCKII (Paired Thr249), Non-Phospho
Reactivity
Human, Mouse, Rat, Chicken, Bovine, Canine
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
Rho Kinase/ROCKII (Paired T249); Rabbit anti ROCKII (Paired Thr249); Non-Phospho; Rabbit anti-ROCKII (Paired T249) Non-phosphospecific; Rho Kinase; Rho-associated kinase-alpha (ROKalpha); Rho-associated; coiled-coil containing protein kinase II (ROCKII); anti-Rho Kinase/ROCKII (Paired T249) antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken, Bovine, Canine
Specificity
This antibody recognizes ~165 kDa of human ROCKII without a phosphorylation site ofThr249. It does not react to phosphorylated protein. This antibody also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
Size: 100 ug/200 ul (varies by lot)
Applicable Applications for anti-Rho Kinase/ROCKII (Paired T249) antibody
ELISA, Western Blot, Immunohistochemistry
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the epitope -CDTAV- without a phosphorylation site Thr396 of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine.
Positive Control
3T3 cell
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Related Product Information for anti-Rho Kinase/ROCKII (Paired T249) antibody
Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
References
Susan Vahebi, et al, Functional Effects of Rho-Kinase-Dependent Phosphorylation of Specific Sites on Cardiac Troponin. Circulation Research. 2005;96:740-747.
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Product Notes
The Rho Kinase/ROCKII (Paired T249) (Catalog #AAA462123) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti ROCKII (Paired Thr249), Non-Phospho reacts with Human, Mouse, Rat, Chicken, Bovine, Canine and may cross-react with other species as described in the data sheet. AAA Biotech's Rho Kinase/ROCKII (Paired T249) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 0.5-2 ug/ml. Researchers should empirically determine the suitability of the Rho Kinase/ROCKII (Paired T249) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rho Kinase/ROCKII (Paired T249), Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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