Testing Data
Rabbit Rho (pS188) Polyclonal Antibody | anti-Rho antibody
Rabbit anti Rho (pS188)
Reactivity
Human, rat, mouse
Applications
ELISA, Western Blot
Purity
The rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Synonyms
Rho (pS188); Polyclonal Antibody; Rabbit anti Rho (pS188); Rabbit anti Rho (pS188) Phospho-specific antibody; Ras homolog gene family; member A variant (RhoA); ARHA; RhoH12; ; anti-Rho antibody
Host
Rabbit
Reactivity
Human, rat, mouse
Clonality
Polyclonal
Specificity
This antibody recognizes ~24 kDa of human RhoA protein. It also reacts with mouse and rat. The other species are not tested.
Purity/Purification
The rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Form/Format
This affinity purified antibody is supplied in sterile Tris-buffered saline (pH7.2) containing antibody stabilizer
Concentration
100 ug/200 ul (varies by lot)
Applicable Applications for anti-Rho antibody
ELISA, Western Blot
Application Notes
Suggested Dilution:
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 2-5 ug/ml
Antigen Preparation
A synthetic peptide corresponding to the C-term of epitope KKKSG at a phosphorylation Ser188 of human Rho protein
Positive Control
MCF7 Cell Lysate
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C to 8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Testing Data
Testing Data
Testing Data 2
Testing Data 2
Related Product Information for anti-Rho antibody
Ras homolog gene family consists of RhoA, RhoB, RhoC proteins. These proteins interact directly with protein kinase (Rho kinase) to serve as downstream effectors of the activated GTPase. The Rho proteins are involved in the regulation of the actin cytosekeleton. Activation of Rho to the GTP bound state promotes formation of stress fibers and is required for the formation and maintenance of focal adhesions. Pathways stimulated by cytokines, hormones and various forms of stress utilize p21 proteins of the Rho family2. Possible mediators for these responses include protein kinase such as p120PKN, and p150, ROK alpha /Rho-A kinase, myosin light chain (MLC), phosphatase and PIP 5-kinase.3
References
• Zavzavadjian,J.R, Mol. Cell Proteomics 6 (3), 413-424 (2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~24 kDa
NCBI Official Full Name
Rho
NCBI Official Symbol
rho
NCBI Protein Information
Transcription termination factor Rho
Protein Family
Similar Products
Product Notes
The Rho (Catalog #AAA462121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Rho (pS188) reacts with Human, rat, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Rho (pS188) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suggested Dilution: Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 2-5 ug/ml. Researchers should empirically determine the suitability of the Rho for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rho (pS188), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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