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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GDA Antibody (MBS9126684) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Rabbit GDA Antibody | anti-GDA antibody

GDA Polyclonal Antibody

Gene Names
GDA; CYPIN; GUANASE; NEDASIN
Applications
Western Blot
Purity
Affinity purification
Synonyms
GDA; GDA Polyclonal Antibody; CYPIN; GUANASE; NEDASIN; GAH; anti-GDA antibody
Ordering
For Research Use Only!
Host
Rabbit
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH 7.3
Applicable Applications for anti-GDA antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Species Reactivity/
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 165-454 of human GDA (NP_004284.1).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using GDA Antibody (MBS9126684) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using GDA Antibody (MBS9126684) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Immunofluorescence (IF)

(Immunofluorescence analysis of A549 cells using GDA antibody (MBS9126684) Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells using GDA antibody (MBS9126684) Blue: DAPI for nuclear staining.)
Related Product Information for anti-GDA antibody
This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-GDA antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed MW: 51kDa
Calculated MW: 42kDa/51kDa/52kDa
NCBI Official Full Name
Guanine deaminase
NCBI Official Synonym Full Names
guanine deaminase
NCBI Official Symbol
GDA
NCBI Official Synonym Symbols
CYPIN; GUANASE; NEDASIN
NCBI Protein Information
guanine deaminase; GAH; p51-nedasin; guanine aminase; guanine aminohydrolase; cytoplasmic PSD95 interactor
UniProt Protein Name
Guanine deaminase
UniProt Gene Name
GDA
UniProt Synonym Gene Names
KIAA1258; Guanase; Guanine aminase; GAH
UniProt Entry Name
GUAD_HUMAN

NCBI Description

This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

GDA: Catalyzes the hydrolytic deamination of guanine, producing xanthine and ammonia. Belongs to the ATZ/TRZ family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleotide Metabolism - purine; EC 3.5.4.3; Hydrolase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: intracellular; cytosol

Molecular Function: guanine deaminase activity; zinc ion binding

Biological Process: nervous system development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; purine nucleotide catabolic process; guanine catabolic process; purine base metabolic process

Research Articles on GDA

Similar Products

Product Notes

The GDA gda (Catalog #AAA9126684) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GDA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the GDA gda for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GDA, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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