Persephin (PSPN) Recombinant Protein | PSPN recombinant protein

Persephin (PSPN)

Cat. Num. AAA517484

• Gene Names: PSPN; PSP
• Applications: Cell Assay
• Synonyms: Persephin (PSPN); PSPN recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)

• Applicable Applications for PSPN recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Sample Cell Assay Data: The ED50 is 4.0-16.0ng/mL as determined by a cell proliferation assay
• Reconstitution: Reconstitute in sterile 4mM HCl at 100ug/mL (containing at least 0.1% human or bovine serum albumin)
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human Persephin (PSPN) resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250.)

Related Product Information for PSPN recombinant protein

Recombinant Human Persephin (PSPN) was expressed in E Coli cells. The protein accession number is O60542
Persephin is a disulfide-linked homodimeric neurotrophic factor belonging to the Transforming Growth Factor-beta (TGF-beta) superfamily and the GDNF sub-family. One cysteine residue, out of the seven conserved, is used for inter-chain disulfide bridging while the others form a cysteine knot ring formation. It signals through RET and one of the four GFR-alpha (alpha-1, alpha-2, alpha-3, alpha-4) receptors to promote the proper development and maintenance of the nervous system. Persephin promotes survival of dopaminergic neurons in the midbrain as well as some motor neurons, and promotes branching of ureteric buds.

Product Categories/Family for PSPN recombinant protein

Growth Factors; Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 2935710
• NCBI GeneID: 5623
• UniProt Accession #: O60542
• Molecular Weight: 16,600 Da
• NCBI Official Full Name: persephin
• NCBI Official Synonym Full Names: persephin
• NCBI Official Symbol: PSPN
• NCBI Official Synonym Symbols: PSP
• NCBI Protein Information: persephin
• UniProt Protein Name: Persephin
• Protein Family: Persephin
• UniProt Gene Name: PSPN
• UniProt Synonym Gene Names: PSP
• UniProt Entry Name: PSPN_HUMAN

NCBI Description

The protein encoded by this gene is a neurotrophic factor, belonging to the GDNF family. Neurotrophic factors are important for the proper development and maintenance of the nervous system. These factors promote neuronal survival and can prevent the neuronal degeneration associated with injury, toxin exposure, or neurodegenerative disease. The encoded protein has amino acid similarity to its other family members, glial cell line-derived neurotrophic factor and neurturin. This gene product promotes the survival of ventral midbrain dopaminergic neurons in culture and prevents their degeneration after 6-hydroxydopamine treatment in vivo. [provided by RefSeq, Jul 2008]

Uniprot Description

PSPN: Exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. Belongs to the TGF-beta family. GDNF subfamily.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: extracellular space

Molecular Function: growth factor activity; receptor binding

Biological Process: axon guidance; nervous system development; central nervous system development; ureteric bud branching