Rabbit DAPK2 Antibody | anti-DAPK2 antibody

Anti-DAP Kinase 2 Antibody

Cat. Num. AAA194056

• Gene Names: DAPK2; DRP1; DRP-1; MGC119312
• Applications: Immunoblot, Western Blot
• Synonyms: DAPK2; Anti-DAP Kinase 2 Antibody; Anti-DAPK2; DAP Kinase 2; anti-DAPK2 antibody

• Host: Rabbit
• Specificity: Anti-DAPK2 [Rabbit antibody to peptide corresponding to aa 356-370 of human DAP Kinase 2. Recognizes human, mouse and rat DAP Kinase 2.]; This antibody recognizes human, mouse, and rat DAPK2 (approx. 42 kD). No crossreactivity with DAPK.
• Form/Format: 100 ug IgG in 200 ul PBS, pH 7.4, purified by immunoaffinity chromatography.

• Applicable Applications for anti-DAPK2 antibody: Immunoblot
• Application Notes: Immunoblotting: use at 1:500-1:1,000 dilution.; Positive control: Whole cell lysate from A431cells.
• Antigen: Peptide corresponding to aa 356- 370 of human DAPK2. This sequence is identical to that of mouse.
• Stabilizer: None
• Preservatives: 0.02% sodium azide.
• Dilutions Instructions: Dilute in PBS or medium which is identical to that used in the assay system.
• Preparation and Storage: This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.

Related Product Information for anti-DAPK2 antibody

Apoptosis is mediated by death domaincontaining adapter molecules and the caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is a proapoptotic, calcium-regulated serine/threonine kinase containing a death domain. Expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFN gamma -induced apoptosis. A DAPK-related protein was recently described and designated DAPK2, or DRP-1. Expression of DAPK2 induces apoptosis. The messenger RNA for DAPK2 is expressed in multiple human tissues.

Product Categories/Family for anti-DAPK2 antibody

Apoptosis Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 14670383
• NCBI GeneID: 23604
• NCBI Accession #: NP_055141.2
• NCBI GenBank Nucleotide #: NM_014326.3
• UniProt Accession #: Q9UIK4; O75892; Q24JS1; Q1RMF4
• Molecular Weight: 42,898 Da
• NCBI Official Full Name: death-associated protein kinase 2
• NCBI Official Synonym Full Names: death-associated protein kinase 2
• NCBI Official Symbol: DAPK2
• NCBI Official Synonym Symbols: DRP1; DRP-1; MGC119312
• NCBI Protein Information: death-associated protein kinase 2; DAP kinase 2; OTTHUMP00000163874; DAP-kinase-related protein 1 beta isoform
• UniProt Protein Name: Death-associated protein kinase 2
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK2
• UniProt Entry Name: DAPK2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq]

Uniprot Description

DAPK2: an ubiquitously expressed protein kinase of the DAPK family. Activated by Ca(2+)/calmodulin. Contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain. Overexpression induces apoptosis. Expressed at high levels in heart, lung and skeletal muscle.

Protein type: EC 2.7.11.1; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CAMK group; DAPK family

Chromosomal Location of Human Ortholog: 15q22.31

Cellular Component: cytoplasm; cytoplasmic vesicle

Molecular Function: calmodulin binding; protein serine/threonine kinase activity; identical protein binding; protein binding; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; regulation of autophagy; protein amino acid phosphorylation; anoikis