RAB6A recombinant protein

RAB6A Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121990

• Gene Names: RAB6A; RAB6
• Purity: >85% as determined by SDS-PAGE
• Synonyms: RAB6A; RAB6A Protein; Human; Recombinant (His Tag); Human Rab6/Rab GTPases/RAB6A Protein (His Tag); RAB6 Protein; member RAS oncogene family; RAB6A recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys208

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human RAB6A (P20340-2)(Met1-Cys208) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RAB6A recombinant protein

Background: Rab6 is one of the most conserved Rab GTPaes throughout evolution and the most abundant Rab protein associated with the Golgi complex. The two ubiquitous Rab isoforms, Rab6A and Rab6A', that are generated by alternative splicing of the RAB6A gene, regulate several transport steps at the Golgi level, including retrograde transport between endosomes and Golgi, anterograde transport between Golgi and the plasma membrane, and intra-Golgi and Golgi to endoplasmic reticulum transport. In MEF cells, most of the functions were attributed to the two ubiquitous Rab6 isoforms.

Product Categories/Family for RAB6A recombinant protein

Autophagy Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 344030202
• NCBI GeneID: 5870
• NCBI Accession #: NP_001230647.1
• NCBI GenBank Nucleotide #: NM_001243718.1
• UniProt Accession #: P20340; Q1W5D8; Q5U0A8; Q9UBE4; A8K133
• Molecular Weight: 23,593 Da
• NCBI Official Full Name: ras-related protein Rab-6A isoform d
• NCBI Official Synonym Full Names: RAB6A, member RAS oncogene family
• NCBI Official Symbol: RAB6A
• NCBI Official Synonym Symbols: RAB6
• NCBI Protein Information: ras-related protein Rab-6A; Rab GTPase; RAB6, member RAS oncogene family
• UniProt Protein Name: Ras-related protein Rab-6A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB6A
• UniProt Synonym Gene Names: RAB6; Rab-6
• UniProt Entry Name: RAB6A_HUMAN

NCBI Description

This gene encodes a member of the RAB family, which belongs to the small GTPase superfamily. GTPases of the RAB family bind to various effectors to regulate the targeting and fusion of transport carriers to acceptor compartments. This protein is located at the Golgi apparatus, which regulates trafficking in both a retrograde (from early endosomes and Golgi to the endoplasmic reticulum) and an anterograde (from the Golgi to the plasma membrane) directions. Myosin II is an effector of this protein in these processes. This protein is also involved in assembly of human cytomegalovirus (HCMV) by interacting with the cellular protein Bicaudal D1, which interacts with the HCMV virion tegument protein, pp150. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

RAB6A: Protein transport. Regulator of membrane traffic from the Golgi apparatus towards the endoplasmic reticulum (ER). Has a low GTPase activity. Interacts with CCDC64; leads to its accumulation in the pericentrosomal region. Interacts with SCYL1BP1. Interacts with VSP52 and RABGAP1. Interacts with GCC2 (via its GRIP domain). Interacts with RAB6IP1 (via its RUN 1 domain). Isoform 1 interacts with RAB6KIFL. Isoform 2 does not interact with RAB6KIFL. Isoform 1 interacts with BICD1. Isoform 2 interacts with BICD1. Isoform 1 interacts with BICD2. Isoform 2 interacts with BICD2. Interacts with TMF1. Isoform 1 (GTP-bound) interacts with DYNLRB1; the interaction is direct. Isoform 2 (GDP-bound) interacts with DYNLRB1; the interaction is direct. Interacts with PIFO/C1orf88. Ubiquitous. Belongs to the small GTPase superfamily. Rab family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: Golgi membrane; Golgi apparatus; membrane; trans-Golgi network; cytoplasmic vesicle; cytosol

Molecular Function: GTPase activity; protein domain specific binding; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: antigen processing and presentation; protein localization in Golgi apparatus; retrograde vesicle-mediated transport, Golgi to ER; peptidyl-cysteine methylation; protein targeting to Golgi; Rab protein signal transduction; retrograde transport, endosome to Golgi