Rab5 Blocking Peptide | RAB5A blocking peptide

Rab5 Antibody (C-term) Blocking Peptide

• Gene Names: RAB5A; RAB5
• Synonyms: Rab5; Rab5 Antibody (C-term) Blocking Peptide; Ras-related protein Rab-5A; RAB5A; RAB5; RAB5A blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of human Rab5. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 215

• Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Early endosome membrane; Lipid-anchor. Melanosome Cytoplasmic vesicle. Cell projection, ruffle. Membrane. Cytoplasm, cytosol. Note: Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RAB5A blocking peptide

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension.

NCBI and Uniprot Product Information

• NCBI GI #: 1346958
• NCBI GeneID: 5868
• UniProt Accession #: P20339; Q6FI44; B4DJA5
• Molecular Weight: 22,178 Da
• NCBI Official Full Name: Ras-related protein Rab-5A
• NCBI Official Synonym Full Names: RAB5A, member RAS oncogene family
• NCBI Official Symbol: RAB5A
• NCBI Official Synonym Symbols: RAB5
• NCBI Protein Information: ras-related protein Rab-5A
• UniProt Protein Name: Ras-related protein Rab-5A
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB5A
• UniProt Synonym Gene Names: RAB5
• UniProt Entry Name: RAB5A_HUMAN

Uniprot Description

RAB5A: Required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, RABEP1, SUN2, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3. Interacts with PIK3CB. Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; G protein, monomeric, Rab; Motility/polarity/chemotaxis; G protein, monomeric

Chromosomal Location of Human Ortholog: 3p24-p22

Cellular Component: axon; cell soma; cytoplasm; cytosol; dendrite; early endosome; endocytic vesicle; endosome; endosome membrane; nerve terminal; plasma membrane; synaptic vesicle; terminal button

Molecular Function: GDP binding; GTP binding; GTPase activity; protein binding

Biological Process: blood coagulation; early endosome to late endosome transport; endocytosis; positive regulation of exocytosis; regulation of endocytosis; regulation of endosome size; regulation of filopodium formation