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Western Blot (WB) (Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 7H4 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10ug (A549, MEF); 20ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody at 1:1000.)

Mouse anti-Human, Mouse RAB1A Monoclonal Antibody | anti-RAB1A antibody

RAB1A Antibody: ATTO 488

Gene Names
RAB1A; RAB1; YPT1
Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Synonyms
RAB1A; Monoclonal Antibody; RAB1A Antibody: ATTO 488; Mouse Anti-Human RAB1A Monoclonal IgG1; Clone 7H4: ATTO 488; GTP binding protein RAB 1A antibody; antibody; RAB 1 antibody; Rab 1A antibody; Rab1A antibody; RAB1A member RAS oncogene family antibody; RAB1A_HUMAN antibody; Ras related protein Rab 1A antibody; Ras-related protein Rab-1A antibody; YPT1-related protein antibody; RAB1 Antibody; anti-RAB1A antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H4
Specificity
Detects ~23kDa. Cross-reacts with RAB1B.
Purity/Purification
Protein G Purified
Form/Format
PBS pH7.4, 50% glycerol, 0.09% Sodium Azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-RAB1A antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
WB: 1:1000
A 1:1000 dilution was sufficient for detection of RAB1A in 10ug (A549, MEF); 20ug (Mouse Brain) lysates by ECL immunoblot analysis.
Immunogen
Full length recombinant RAB1A protein
Immunogen Species
Human
Conjugation
ATTO 488
Tissue Specificity
Low tissue specificity.
Cellular Localization
Cytoplasm, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane
Preparation and Storage
Store at -20 degree C
Shipping: Blue Ice

Western Blot (WB)

(Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 7H4 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10ug (A549, MEF); 20ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody at 1:1000.)

Western Blot (WB) (Western Blot analysis of Human, Mouse A549 WT and KO cells, MEF, mouse brain cells showing detection of RAB1A protein using Mouse Anti-RAB1A Monoclonal Antibody, Clone 7H4 . Lane 1: MW ladder. Lane 2: WT A549 cells. Lane 3-5: KO A549 clones. Lane 6: MEF cells. Lane 7: mouse brain cells.. Load: 10ug (A549, MEF); 20ug (Mouse Brain). Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody at 1:1000.)

Immunoprecipitation (IP0

(Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 7H4 . Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody for 2 hours..)

Immunoprecipitation (IP0 (Immunoprecipitation analysis using Mouse Anti-RAB1A Monoclonal Antibody, Clone 7H4 . Tissue: HEK293 cells overexpressing RAB1A, RAB1B, and RAB1C. Species: Human. Primary Antibody: Mouse Anti-RAB1A Monoclonal Antibody for 2 hours..)
Related Product Information for anti-RAB1A antibody
Rab1A is mostly found in the membrane of the endoplasmic reticulum (ER) and Golgi apparatus and is involved in cell signaling, cancer, and neurodegenerative diseases (1). It regulates protein transport from the ER to the Golgi compartment and is involved in growth hormone secretion (2).
References
1. Yang, X. et al. (2016) Oncogene. 35(44):5699-57042. pubchem.ncbi.nlm.nih.gov/protein/P62820

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,903 Da
NCBI Official Full Name
ras-related protein Rab-1A isoform 1
NCBI Official Synonym Full Names
RAB1A, member RAS oncogene family
NCBI Official Symbol
RAB1A
NCBI Official Synonym Symbols
RAB1; YPT1
NCBI Protein Information
ras-related protein Rab-1A; GTP binding protein Rab1a; RAB1, member RAS oncogene family; Rab GTPase YPT1 homolog; YPT1-related protein
UniProt Protein Name
Ras-related protein Rab-1A
Protein Family
UniProt Gene Name
RAB1A
UniProt Synonym Gene Names
RAB1
UniProt Entry Name
RAB1A_HUMAN

NCBI Description

This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

RAB1A: Probably required for transit of protein from the ER through Golgi compartment. Binds GTP and GDP and possesses intrinsic GTPase activity. Belongs to the small GTPase superfamily. Rab family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein, monomeric; G protein, monomeric, Rab; G protein

Chromosomal Location of Human Ortholog: 2p14

Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; early endosome; melanosome; cytosol

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding

Biological Process: ER to Golgi vesicle-mediated transport; cell migration; endocytosis; virus assembly; melanosome transport; vesicle-mediated transport; growth hormone secretion; intracellular protein transport; defense response to bacterium; vesicle transport along microtubule; autophagy; cilium biogenesis; mitotic cell cycle; Rab protein signal transduction; Golgi organization and biogenesis; autophagic vacuole formation

Research Articles on RAB1A

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Product Notes

The RAB1A rab1a (Catalog #AAA8005913) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The RAB1A Antibody: ATTO 488 reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RAB1A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000 A 1:1000 dilution was sufficient for detection of RAB1A in 10ug (A549, MEF); 20ug (Mouse Brain) lysates by ECL immunoblot analysis. Researchers should empirically determine the suitability of the RAB1A rab1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB1A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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