Typical Standard Curve/Testing Data
Human Putative peptide YY-2 ELISA Kit | PYY2 elisa kit
Human Putative peptide YY-2 ELISA Kit
Reactivity
Human
Synonyms
Putative peptide YY-2; Human Putative peptide YY-2 ELISA Kit; PYY2/Putative peptide YY-2/Putative peptide YY2; PYY2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PYY2. No significant cross-reactivity or interference between PYY2 and analogues was observed.
Sequence Length
33
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
15.6-1000pg/ml
Sensitivity
<9.375pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PYY2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PYY2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for PYY2 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PYY2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PYY2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PYY2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PYY2 can be calculated.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
3,432 Da
NCBI Official Full Name
Putative peptide YY-2
UniProt Protein Name
Putative peptide YY-2
Protein Family
UniProt Gene Name
PYY2
UniProt Synonym Gene Names
Putative peptide YY2
UniProt Entry Name
PYY2_HUMAN
Uniprot Description
PYY2: Belongs to the NPY family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q11
Cellular Component: extracellular space
Molecular Function: G-protein-coupled receptor binding; neuropeptide hormone activity
Biological Process: cell-cell signaling; digestion; feeding behavior; neuropeptide signaling pathway; regulation of appetite
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Product Notes
The Human PYY2 pyy2 (Catalog #AAA765706) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA765706 ELISA Kit recognizes Human PYY2. It is sometimes possible for the material contained within the vial of "Putative peptide YY-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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