Human H2AFX (Histone H2AX) ELISA Kit | H2AFX elisa kit

Human H2AFX (Histone H2AX) ELISA Kit

Cat. Num. AAA7607016

• Gene Names: H2AFX; H2AX; H2A.X; H2A/X
• Reactivity: Human
• Synonyms: H2AFX (Histone H2AX); Human H2AFX (Histone H2AX) ELISA Kit; H2AFX elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Gzmb. No significant cross-reactivity or interference between Gzmb and analogues was observed.
• Sequence Length: 143

• Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.6-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Gzmb were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Gzmb were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for H2AFX elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Gzmb antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Gzmb antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Gzmb amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Gzmb can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4504253
• NCBI GeneID: 3014
• NCBI Accession #: NP_002096.1
• NCBI GenBank Nucleotide #: NM_002105.2
• UniProt Accession #: P16104; Q4ZGJ7; Q6IAS5
• Molecular Weight: 15,145 Da
• NCBI Official Full Name: histone H2AX
• NCBI Official Synonym Full Names: H2A histone family member X
• NCBI Official Symbol: H2AFX
• NCBI Official Synonym Symbols: H2AX; H2A.X; H2A/X
• NCBI Protein Information: histone H2AX
• UniProt Protein Name: Histone H2AX
• UniProt Gene Name: H2AFX
• UniProt Synonym Gene Names: H2AX; H2a/x

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015]

Uniprot Description

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.