Canine Peptide YY ELISA Kit | PYY elisa kit

Canine Peptide YY ELISA Kit

Cat. Num. AAA735000

• Gene Names: PYY; PYY1; PYY-I
• Reactivity: Canine
• Synonyms: Peptide YY; Canine Peptide YY ELISA Kit; PYY elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This PYY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PYY. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PYY elisa kit

Principle of the assay: PYY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PYY antibody and an PYY-HRP conjugate. The assay sample and buffer are incubated together with PYY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PYY concentration since PYY from samples and PYY-HRP conjugate compete for the anti-PYY antibody binding site. Since the number of sites is limited, as more sites are occupied by PYY from the sample, fewer sites are left to bind PYY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PYY concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PYY elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 49457254
• NCBI GeneID: 5697
• UniProt Accession #: P10082; Q5U5Q6; Q6FGH8
• Molecular Weight: 10,360 Da
• NCBI Official Full Name: PYY
• NCBI Official Synonym Full Names: peptide YY
• NCBI Official Symbol: PYY
• NCBI Official Synonym Symbols: PYY1; PYY-I
• NCBI Protein Information: peptide YY; prepro-PYY; peptide tyrosine tyrosine
• UniProt Protein Name: Peptide YY
• Protein Family: Peptide
• UniProt Gene Name: PYY
• UniProt Synonym Gene Names: PYY
• UniProt Entry Name: PYY_HUMAN

NCBI Description

The protein encoded by this gene is proteolytically processed to release a peptide that inhibits pancreatic secretion and mobility in the gut. Rare variations in this gene could increase susceptibility to obesity. [provided by RefSeq, Jul 2010]

Uniprot Description

PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; protein binding; G-protein-coupled receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; cell-cell signaling; eating behavior; neuropeptide signaling pathway; regulation of appetite; digestion; cytoskeleton organization and biogenesis; feeding behavior; cell motility

Disease: Obesity