Mouse anti-Human HSPB7 Monoclonal Antibody | anti-HSPB7 antibody

HSPB7 (Heat Shock Protein beta-7, Cardiovascular Heat Shock Protein, cvHsp, CVHSP, DKFZp779D0968, FLJ32733)

Cat. Num. AAA6001591

• Gene Names: HSPB7; cvHSP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: HSPB7; Monoclonal Antibody; HSPB7 (Heat Shock Protein beta-7; Cardiovascular Heat Shock Protein; cvHsp; CVHSP; DKFZp779D0968; FLJ32733); Anti -HSPB7 (Heat Shock Protein beta-7; anti-HSPB7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3E11
• Specificity: Recognizes human HSPB7.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSHRTSSTFRAERSFHSSSSSSSSSTSSSASRALPAQDPPMEKALSMFSDDFGSFMRPHSEPLAFPARPGGAGNIKTLGDAYEFAVDVRDFSPEDIIVTTSNNHIEVRAEKLAADGTVMNTFAHKCQLPEDVDPTSVTSALREDGSLTIRARRHPHTEHVQQTFRTEIKI*

• Applicable Applications for anti-HSPB7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 3ug/ml
• Immunogen: Full length recombinant corresponding to aa1-171 from human HSPB7 (AAH06319) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (44.81kD).)

Western Blot (WB)

(HSPB7 monoclonal antibody. Western Blot analysis of HSPB7 expression in human colon.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to HSPB7 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged HSPB7 is ~0.1ng/ml as a capture antibody.)

Related Product Information for anti-HSPB7 antibody

HSPB7 is a member of HSPB (Heat shock protein beta) family. The HSPB family is one of the more diverse families within the group of HSP families. Some members have chaperone-like activities and/or play a role in cytoskeletal stabilization. Some members also show a dynamic, stress-induced translocation to SC35 splicing speckles. HSPB7 constitutively localized to SC35 splicing speckles, driven by its N-terminus. Unlike HSPB1 and HSPB5, that chaperoned heat unfolded substrates and kept them folding competent, HSPB7 did not support refolding. Recombinant HSPB7 protein was expressed in E. coli.

Product Categories/Family for anti-HSPB7 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 7657202
• NCBI GeneID: 27129
• NCBI Accession #: NP_055239.1
• NCBI GenBank Nucleotide #: NM_014424.4
• UniProt Accession #: Q9UBY9; Q9NU17; B3KQ37; C9K0Y0
• Molecular Weight: 18,611 Da
• NCBI Official Full Name: heat shock protein beta-7
• NCBI Official Synonym Full Names: heat shock 27kDa protein family, member 7 (cardiovascular)
• NCBI Official Symbol: HSPB7
• NCBI Official Synonym Symbols: cvHSP
• NCBI Protein Information: heat shock protein beta-7; cardiovascular heat shock protein; heat shock 27kD protein family, member 7 (cardiovascular)
• UniProt Protein Name: Heat shock protein beta-7
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB7
• UniProt Synonym Gene Names: CVHSP; HspB7; cvHsp
• UniProt Entry Name: HSPB7_HUMAN

Uniprot Description

HSPB7: Belongs to the small heat shock protein (HSP20) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: Cajal body; mitochondrion; cytoplasm; nucleus; actin cytoskeleton

Molecular Function: protein C-terminus binding; protein binding; filamin binding

Biological Process: regulation of heart contraction; response to unfolded protein