Rabbit PYGL Polyclonal Antibody | anti-PYGL antibody

PYGL Polyclonal Antibody

Cat. Num. AAA2520774

• Gene Names: PYGL; GSD6
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: PYGL; Polyclonal Antibody; PYGL Polyclonal Antibody; GSD6; anti-PYGL antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 2mg/mL (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-PYGL antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:30-1:150
• Immunogen: Recombinant protein of human PYGL
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of 231 and PC3 cell using PYGL Polyclonal Antibody at dilution of 1:400)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human thyroid cancer using PYGL Polyclonal Antibody at dilution of 1:35)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human brain using PYGL Polyclonal Antibody at dilution of 1:35)

Related Product Information for anti-PYGL antibody

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1, 4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively.

NCBI and Uniprot Product Information

• NCBI GI #: 40675618
• NCBI GeneID: 5836
• Molecular Weight: 93,134 Da
• NCBI Official Full Name: PYGL protein, partial
• NCBI Official Synonym Full Names: phosphorylase, glycogen, liver
• NCBI Official Symbol: PYGL
• NCBI Official Synonym Symbols: GSD6
• NCBI Protein Information: glycogen phosphorylase, liver form
• UniProt Protein Name: Glycogen phosphorylase, liver form
• Protein Family: Glycogen phosphorylase
• UniProt Gene Name: PYGL
• UniProt Entry Name: PYGL_HUMAN

NCBI Description

This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

PYGL: Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). A metabolic disorder characterized by mild to moderate hypoglycemia, mild ketosis, growth retardation, and prominent hepatomegaly. Heart and skeletal muscle are not affected. Belongs to the glycogen phosphorylase family.

Protein type: EC 2.4.1.1; Carbohydrate Metabolism - starch and sucrose; Transferase

Chromosomal Location of Human Ortholog: 14q21-q22

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: glycogen phosphorylase activity; protein homodimerization activity; bile acid binding; purine binding; drug binding; vitamin binding; ATP binding; AMP binding; pyridoxal phosphate binding; glucose binding

Biological Process: glycogen metabolic process; glycogen catabolic process; carbohydrate metabolic process; 5-phosphoribose 1-diphosphate biosynthetic process; glucose metabolic process; pathogenesis; glucose homeostasis

Disease: Glycogen Storage Disease Vi