Pyrroline-5-Carboxylate Reductase 1 Recombinant Protein | PYCR1 recombinant protein

Recombinant Human Pyrroline-5-Carboxylate Reductase 1

Cat. Num. AAA144208

• Gene Names: PYCR1; P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Pyrroline-5-Carboxylate Reductase 1; Recombinant Human Pyrroline-5-Carboxylate Reductase 1; PYCR1 Human; Pyrroline-5-Carboxylate Reductase 1 Human Recombinant; P5C; PRO3; P5CR 1; Pyrroline-5-Carboxylate Reductase 1 mitochondrial; ARCL2B; PYCR; PIG45; PP222; Proliferation-Inducing Protein 45; PYCR1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: The PYCR1 protein solution (0.5mg/1ml) is formulated in 20 mM Tris-HCl Buffer (pH 8.5) and 10% Glycerol.; Sterile Filtered clear solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSVGFIGAGQ LAFALAKGFT AAGVLAAHKI MASSPDMDLA TVSALRKMGV KLTPHNKETV QHSDVLFLAV KPHIIPFILD EIGADIEDRH IVVSCAAGVT ISSIEKKLSA FRPAPRVIRC MTNTPVVVRE GATVYATGTH AQVEDGRLME QLLSSVGFCT EVEEDLIDAV TGLSGSGPAY AFTALDALAD GGVKMGLPRR LAVRLGAQAL LGAAKMLLHS EQHPGQLKDN VSSPGGATIH ALHVLESGGF RSLLINAVEA SCIRTRELQS MADQEQVSPA AIKKTILDKV KLDSPAGTAL SPSGHTKLLP RSLAPAGKD
• Sequence Length: 319

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PYCR1 recombinant protein

Description: PYCR1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 339 amino acids (1-319a.a.) and having a molecular mass of 35.5kDa.PYCR1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: PYCR1 is a universal housekeeping enzyme which catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. PYCR1 enzyme also takes a physiologic part in the generation of NADP(+) in certain cell types. PYCR1 forms a homopolymer and localizes to the mitochondrion. Mutations in PYCR1 are the source of cutis laxa autosomal recessive type 2B (ARCL2B).

Product Categories/Family for PYCR1 recombinant protein

Enzymes; Reductase

NCBI and Uniprot Product Information

• NCBI GI #: 533112488
• NCBI GeneID: 5831
• NCBI Accession #: NP_001269209.1
• NCBI GenBank Nucleotide #: NM_001282280.1
• UniProt Accession #: P32322; Q6FHI4; Q96DI6; Q9HBQ4; A6NFM2; B4DMU0
• Molecular Weight: 35,981 Da
• NCBI Official Full Name: pyrroline-5-carboxylate reductase 1, mitochondrial isoform 1
• NCBI Official Synonym Full Names: pyrroline-5-carboxylate reductase 1
• NCBI Official Symbol: PYCR1
• NCBI Official Synonym Symbols: P5C; P5CR; PRO3; PYCR; PIG45; PP222; ARCL2B; ARCL3B
• NCBI Protein Information: pyrroline-5-carboxylate reductase 1, mitochondrial; P5C reductase 1; proliferation-inducing protein 45
• UniProt Protein Name: Pyrroline-5-carboxylate reductase 1, mitochondrial
• Protein Family: Pyrroline-5-carboxylate reductase
• UniProt Gene Name: PYCR1
• UniProt Synonym Gene Names: P5C reductase 1; P5CR 1
• UniProt Entry Name: P5CR1_HUMAN

NCBI Description

This gene encodes an enzyme that catalyzes the NAD(P)H-dependent conversion of pyrroline-5-carboxylate to proline. This enzyme may also play a physiologic role in the generation of NADP(+) in some cell types. The protein forms a homopolymer and localizes to the mitochondrion. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

PYCR1: Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress. Defects in PYCR1 are the cause of cutis laxa autosomal recessive type 2B (ARCL2B). A multisystem disorder characterized by the appearance of premature aging, wrinkled and lax skin with reduced elasticity, joint laxity, craniofacial dysmorphic features, intrauterine growth retardation with some degree of postnatal growth deficiency, and developmental delay. Defects in PYCR1 are the cause of cutis laxa, autosomal recessive, type 3B (ARCL3B). ARCL3B is a disorder characterized by an aged appearance with distinctive facial features, sparse hair, ophthalmologic abnormalities, intrauterine growth retardation, and cutis laxa. Belongs to the pyrroline-5-carboxylate reductase family.

Protein type: EC 1.5.1.2; Mitochondrial; Oxidoreductase; Amino Acid Metabolism - arginine and proline

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function: identical protein binding; protein binding; pyrroline-5-carboxylate reductase activity

Biological Process: regulation of mitochondrial membrane potential; proline biosynthetic process; amino acid biosynthetic process

Disease: Cutis Laxa, Autosomal Recessive, Type Iib; Cutis Laxa, Autosomal Recessive, Type Iiib