Monkey P53 Upregulated Modulator Of Apoptosis ELISA Kit | PUMA elisa kit

Monkey P53 Upregulated Modulator Of Apoptosis ELISA Kit

Cat. Num. AAA744227

• Gene Names: Bbc3; Puma
• Reactivity: Monkey
• Synonyms: P53 Upregulated Modulator Of Apoptosis; Monkey P53 Upregulated Modulator Of Apoptosis ELISA Kit; PUMA elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PUMA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PUMA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PUMA elisa kit

Principle of the Assay: PUMA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PUMA antibody and an PUMA-HRP conjugate. The assay sample and buffer are incubated together with PUMA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PUMA concentration since PUMA from samples and PUMA-HRP conjugate compete for the anti-PUMA antibody binding site. Since the number of sites is limited, as more sites are occupied by PUMA from the sample, fewer sites are left to bind PUMA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PUMA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PUMA elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 27542559
• NCBI GeneID: 317673
• Molecular Weight: 20,718 Da
• NCBI Official Full Name: PUMA
• NCBI Official Synonym Full Names: Bcl-2 binding component 3
• NCBI Official Symbol: Bbc3
• NCBI Official Synonym Symbols: Puma
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: Bbc3
• UniProt Synonym Gene Names: Puma
• UniProt Entry Name: BBC3_RAT

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Cellular Component: mitochondrion; intracellular

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; positive regulation of protein homooligomerization; determination of adult life span; glial cell differentiation; release of sequestered calcium ion into cytosol; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; brain development; response to DNA damage stimulus; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; negative regulation of growth