Rabbit anti-Human, Mouse Integrin alpha 6 Polyclonal Antibody | anti-ITGA6 antibody

Integrin alpha 6 monoclonal antibody

Cat. Num. AAA3006684

• Gene Names: ITGA6; CD49f; VLA-6; ITGA6B
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: Integrin alpha 6; Polyclonal Antibody; Integrin alpha 6 monoclonal antibody; Integrin alpha-6; Alpha6p; VLA 6 alpha subunit; VLA 6; VLA-6; Integrin alpha6; CD49 antigen-like family member F; CD49f; Integrin alpha6B; Integrin; alpha 6; ITA6_HUMAN; ITGA6; ITGA6B; Processed integrin alpha-6; anti-ITGA6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Integrin alpha 6 monoclonal Antibody detects endogenous levels of Integrin alpha 6 protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-ITGA6 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:500-2000; IHC-P: 1:50-200
• Immunogen: Recombinant Human Integrin alpha 6:1-100/1130
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Testing Data

(Anti-Integrin alpha 6 at 1/1000 dilution)

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Integrin alpha 6) Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)

Related Product Information for anti-ITGA6 antibody

Integrins are important extracellular matrix (ECM) receptor proteins located on cell surfaces. They are hetrodimers composed of an alpha and a beta transmembrane glycoprotein subunit. Around twenty two different integrins (different alpha/ beta subunit combinations) are found in nature. Integrins are generally present in high concentrations at the cell surface, but, unlike most other cell surface receptors, they bind ligands with very low affinity. Due to their weak individual binding, integrins need to cluster and bind in groups in order to effectively bind the ECM. Integrins bind many different ligands including laminin. Each integrin is made up of a large N terminal extracellular domain that binds the ECM ligand and a small C terminal cytoplasmic domain that mediates interaction with the actin cytoskeleton and signaling function. Integrin alpha 6 complexes are receptors for laminins, which are components of basement membranes. Integrin alpha 6 complexes may play an important role in embryogenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 119395742
• NCBI GeneID: 3655
• NCBI Accession #: NP_000201.2
• NCBI GenBank Nucleotide #: NM_000210.2
• UniProt Accession #: P23229; Q08443; Q0MRC7; Q14646; Q16508; Q53RX7; Q59HB7; Q86VL6; B2RMU9; B4DG69; B4DKB8; C4AM96
• Molecular Weight: 126,633 Da
• NCBI Official Full Name: integrin alpha-6 isoform b
• NCBI Official Synonym Full Names: integrin, alpha 6
• NCBI Official Symbol: ITGA6
• NCBI Official Synonym Symbols: CD49f; VLA-6; ITGA6B
• NCBI Protein Information: integrin alpha-6; integrin alpha6B; CD49 antigen-like family member F
• UniProt Protein Name: Integrin alpha-6
• Protein Family: Integrin
• UniProt Gene Name: ITGA6
• UniProt Entry Name: ITA6_HUMAN

NCBI Description

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Receptor, misc.; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cell surface; cell-cell adherens junction; focal adhesion; hemidesmosome; plasma membrane; basal plasma membrane; basement membrane; filopodium; external side of plasma membrane

Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; cell-cell adhesion; hemidesmosome assembly; gut development; cellular response to extracellular stimulus; brown fat cell differentiation; positive regulation of cell-cell adhesion; cell-substrate junction assembly; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation

Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia