Protein Tyrosine Phosphatase Receptor Type F Recombinant Protein | PTPRF recombinant protein

Recombinant Protein Tyrosine Phosphatase Receptor Type F (PTPRF)

Cat. Num. AAA2033188

• Gene Names: PTPRF; LAR; BNAH2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protein Tyrosine Phosphatase Receptor Type F; Recombinant Protein Tyrosine Phosphatase Receptor Type F (PTPRF); PTPR-F; LAR; Leukocyte common antigen related; PTPRF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: D SKPVFIKVPE DQTGLSGGVA SFVCQATGEP KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR DEAIYECTAT NSLGEINTSA KLSVLEEEQL PPGFPSIDMG PQLKVVEKAR TATMLCAAGG NPDPEISWFK DFLPVDPATS NGRIKQLRSG ALQIESSEESDQGKYECVAT NSAGTRYSAP ANLY
• Sequence Length: 262

• Applicable Applications for PTPRF recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 24.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Asp30~Tyr224
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 78191531
• NCBI GeneID: 5792
• Molecular Weight: 211,687 Da
• NCBI Official Full Name: protein tyrosine phosphatase receptor type F
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type F
• NCBI Official Symbol: PTPRF
• NCBI Official Synonym Symbols: LAR; BNAH2
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase F
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase F
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRF
• UniProt Synonym Gene Names: LAR; LAR
• UniProt Entry Name: PTPRF_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains three Ig-like domains, and nine non-Ig like domains similar to that of neural-cell adhesion molecule. This PTP was shown to function in the regulation of epithelial cell-cell contacts at adherents junctions, as well as in the control of beta-catenin signaling. An increased expression level of this protein was found in the insulin-responsive tissue of obese, insulin-resistant individuals, and may contribute to the pathogenesis of insulin resistance. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PTPRF: Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase). Belongs to the protein-tyrosine phosphatase family. Receptor class 2A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein tyrosine phosphatase activity; transmembrane receptor protein tyrosine phosphatase activity

Biological Process: cell migration; synaptic transmission; transmembrane receptor protein tyrosine phosphatase signaling pathway

Disease: Breasts And/or Nipples, Aplasia Or Hypoplasia Of, 2