Mouse Tyrosine-protein phosphatase non-receptor type 1 ELISA Kit | Ptpn1 elisa kit

Mouse Tyrosine-protein phosphatase non-receptor type 1 ELISA Kit

Cat. Num. AAA764808

• Gene Names: Ptpn1; PTP1B; PTP-1B; PTP-HA2
• Reactivity: Mouse
• Synonyms: Tyrosine-protein phosphatase non-receptor type 1; Mouse Tyrosine-protein phosphatase non-receptor type 1 ELISA Kit; Ptpn1/Tyrosine-protein phosphatase non-receptor type 1/Protein-tyrosine phosphatase 1B/PTP-1B/PTP1B/protein tyrosine phosphatase 1B/protein tyrosine phosphatase; non-receptor type 1/PTP1Bprotein tyrosine phosphatase; placental; Ptpn1 elisa kit

• Reactivity: Mouse
• Sequence Length: 432

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ptpn1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ptpn1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ptpn1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ptpn1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ptpn1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 133505845
• NCBI GeneID: 19246
• NCBI Accession #: NP_035331.3
• NCBI GenBank Nucleotide #: NM_011201.3
• Molecular Weight: 49,593 Da
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: Ptpn1
• NCBI Official Synonym Symbols: PTP1B; PTP-1B; PTP-HA2
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• Protein Family: Tyrosine-protein phosphatase non-receptor
• UniProt Gene Name: Ptpn1
• UniProt Synonym Gene Names: PTP-1B; PTP-HA2
• UniProt Entry Name: PTN1_MOUSE

Uniprot Description

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: Protein phosphatase, tyrosine (non-receptor); Phosphatase; Motility/polarity/chemotaxis; EC 3.1.3.48

Cellular Component: cytoplasm; cytoplasmic vesicle; cytosol; early endosome; endoplasmic reticulum; membrane; plasma membrane

Molecular Function: enzyme binding; ephrin receptor binding; hydrolase activity; insulin receptor binding; phosphoprotein phosphatase activity; phosphoric monoester hydrolase activity; protein kinase binding; protein phosphatase 2A binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; zinc ion binding

Biological Process: actin cytoskeleton reorganization; activation of JNK activity; dephosphorylation; insulin receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid dephosphorylation; regulation of endocytosis; regulation of insulin receptor signaling pathway; regulation of intracellular protein transport; regulation of signal transduction; unfolded protein response