Mouse Protein Tyrosine Phosphatase Receptor Type N ELISA Kit | PTPRN elisa kit

Mouse Protein Tyrosine Phosphatase Receptor Type N ELISA Kit

Cat. Num. AAA764951

• Gene Names: PTPRN; IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP
• Reactivity: Mouse
• Synonyms: Protein Tyrosine Phosphatase Receptor Type N; Mouse Protein Tyrosine Phosphatase Receptor Type N ELISA Kit; PTPRN/IA2/ICA512/PTPRN/R-PTP-N/CA 512/IA2/IA-2/IA-2/PTP/ICA 512/ICA3/ICA512/islet cell antigen 2/Islet cell antigen 512/Islet cell autoantigen 3/protein tyrosine phosphatase; receptor type; N/protein tyrosine phosphatase-like N/PTP IA-2/receptor-type tyrosine-protein phosphatase-like N/R-PTP-N; PTPRN elisa kit

• Reactivity: Mouse
• Sequence Length: 824

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PTPRN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse PTPRN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse PTPRN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PTPRN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofMouse PTPRN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 62088316
• NCBI GeneID: 5798
• Molecular Weight: 96,261 Da
• NCBI Official Full Name: protein tyrosine phosphatase, receptor type, N variant, partial
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, receptor type N
• NCBI Official Symbol: PTPRN
• NCBI Official Synonym Symbols: IA2; IA-2; ICA512; R-PTP-N; IA-2/PTP
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase-like N
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase-like N
• Protein Family: Receptor-type tyrosine-protein phosphatase
• UniProt Gene Name: PTPRN
• UniProt Synonym Gene Names: ICA3; ICA512; ICA 512
• UniProt Entry Name: PTPRN_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single catalytic domain, and thus represents a receptor-type PTP. This PTP was found to be an autoantigen that is reactive with insulin-dependent diabetes mellitus (IDDM) patient sera, and thus may be a potential target of autoimmunity in diabetes mellitus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Dec 2010]

Uniprot Description

PTPRN: Implicated in neuroendocrine secretory processes. May be involved in processes specific for neurosecretory granules, such as their biogenesis, trafficking or regulated exocytosis or may have a general role in neuroendocrine functions. Seems to lack intrinsic enzyme activity. May play a role in the regulation of secretory granules via its interaction with SNTB2. Belongs to the protein-tyrosine phosphatase family. Receptor class 8 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 2q35-q36.1

Cellular Component: cell junction; cell soma; endosome; Golgi apparatus; integral to membrane; nerve terminal; perikaryon; plasma membrane; secretory granule; synapse; synaptic vesicle

Molecular Function: GTPase binding; protein binding; protein tyrosine phosphatase activity; spectrin binding

Biological Process: cytokine and chemokine mediated signaling pathway; insulin secretion; luteinization; protein amino acid dephosphorylation; response to cAMP; response to estrogen stimulus; response to insulin stimulus; response to reactive oxygen species