Sheep Protein tyrosine phosphatase ELISA Kit | PTP elisa kit

Sheep Protein tyrosine phosphatase ELISA Kit

Cat. Num. AAA752836

• Reactivity: Sheep
• Synonyms: Protein tyrosine phosphatase; Sheep Protein tyrosine phosphatase ELISA Kit; PTP elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This PTP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PTP. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This PTP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PTP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTP elisa kit

Principle of the Assay: PTP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTP antibody and an PTP-HRP conjugate. The assay sample and buffer are incubated together with PTP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTP concentration since PTP from samples and PTP-HRP conjugate compete for the anti-PTP antibody binding site. Since the number of sites is limited, as more sites are occupied by PTP from the sample, fewer sites are left to bind PTP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1479976
• Molecular Weight: 164,613 Da
• NCBI Official Full Name: protein tyrosine phosphatase
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase kappa
• Protein Family: Preterminal protein
• UniProt Gene Name: PTPRK
• UniProt Synonym Gene Names: PTPK; Protein-tyrosine phosphatase kappa; R-PTP-kappa
• UniProt Entry Name: PTPRK_HUMAN

Uniprot Description

PTPRK: a receptor tyrosine phosphatase involved in processes including cell contact and adhesion, growth, tumor invasion, and metastasis. Is a negative regulator of EGFR signaling. Forms complexes with beta-catenin and gamma-catenin, and may modulate the phosphorylation status of beta-catenin. Absent or down-regulated in multiple melanoma cell lines and some melanoma tumors. The gene lies in a region frequently deleted in hematological neoplasms, melanomas, ovary carcinomas, and many other solid tumors. Can play a dual role of tumor suppressor and tumor promoter in mammary epithelial cells. Overexpression results in inhibition of basal proliferation and EGFR signaling in cancer cells. A key regulator of EGFR tyrosine phosphorylation and function in human keratinocytes. TGF-beta 1 stimulates its expression, which may be important for the inhibition of keratinocyte proliferation. Belongs to the protein-tyrosine phosphatase family, receptor class 2B subfamily. Four isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; EC 3.1.3.48; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine

Chromosomal Location of Human Ortholog: 6q22.2-q22.3

Cellular Component: photoreceptor outer segment; adherens junction; cell surface; cell soma; axon; integral to plasma membrane; dendrite; integral to membrane; intercellular junction

Molecular Function: protein binding; gamma-catenin binding; transmembrane receptor protein tyrosine phosphatase activity; beta-catenin binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: focal adhesion formation; negative regulation of cell proliferation; cell migration; transforming growth factor beta receptor signaling pathway; negative regulation of transcription, DNA-dependent; signal transduction; protein amino acid dephosphorylation; cell adhesion; negative regulation of cell migration; negative regulation of cell cycle