Rabbit anti-Human Ube2L6 Polyclonal Antibody | anti-UBE2L6 antibody

Anti-Ube2L6 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106568

• Gene Names: UBE2L6; RIG-B; UBCH8
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Ube2L6; Polyclonal Antibody; Anti-Ube2L6 Antibody; Rabbit Polyclonal; UBCH8/UBE2L6 Antibody; Rabbit PAb; Antigen Affinity Purified; ubiquitin-conjugating enzyme E2L 6; Anti-RIG-B Antibody; Anti-UBCH8 Antibody; anti-UBE2L6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human UBCH8/UBE2L6
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-UBE2L6 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 1-4uL/mg of lysate
• Immunogen: Recombinant Human UBCH8/UBE2L6 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human UBCH8/UBE2L6 (rh UBCH8/UBE2L6). UBCH8/UBE2L6 specific IgG was purified by Human UBCH8/UBE2L6 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-UBE2L6 rabbit polyclonal antibody at 1:500 dilutionLane A: Daudi Whole Cell LysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:18 kDaObserved band size:17 kDa)

Immunoprecipitation (IP)

(UBE2L6 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-UBE2L6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-UBE2L6 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 18 kDaObserved band size: 18 kDa)

Related Product Information for anti-UBE2L6 antibody

UBCH8, also known as UBE2L6, belongs to the ubiquitin-conjugating enzyme family. The family of ubiquitin-conjugating (E2) enzymes is characterized by the presence of a highly conserved ubiquitin-conjugating (UBC) domain. These domains accommodate the ATP-activated ubiquitin (Ub) or ubiquitin-like (UBL) protein via a covalently linked thioester onto its active-site residue. E2 enzymes act via selective protein-protein interactions with the E1 and E3 enzymes and connect activation to covalent modification. By doing so, E2s differentiate effects on downstream substrates, either with a single Ub/UBL molecule or as a chain. UBCH8 is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. It catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. UBCH8 functions in the E6/E6-AP-induced ubiquitination of p53/TP53 and promotes ubiquitination and subsequent proteasomal degradation of FLT3. At protein level, it is present in natural killer cells.

References

Moynihan TP, et al. (1999) The ubiquitin-conjugating enzymes UbcH7 and UbcH8 interact with RING finger/IBR motif-containing domains of HHARI and H7-AP1. J Biol Chem. 274(43):30963-8.Ardley HC, et al. (2000) Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. Cytogenet Cell Genet. 89(1-2):137-40.Zhao C, et al. (2004) The UbcH8 ubiquitin E2 enzyme is also the E2 enzyme for ISG15, an IFN-alpha/beta-induced ubiquitin-like protein. Proc Natl Acad. 101(20):7578-82.Tripathi MK, et al. (2005) Down-regulation of UCRP and UBE2L6 in BRCA2 knocked-down human breast cells. Biochem Biophys Res Commun. 328(1):43-8.

NCBI and Uniprot Product Information

• NCBI GI #: 239938941
• NCBI GeneID: 9246
• UniProt Accession #: O14933; Q8N5D8; Q9UEZ0; A6NDM6; A8MY53
• Molecular Weight: 153
• NCBI Official Full Name: Ubiquitin/ISG15-conjugating enzyme E2 L6
• NCBI Official Synonym Full Names: ubiquitin-conjugating enzyme E2L 6
• NCBI Official Symbol: UBE2L6
• NCBI Official Synonym Symbols: RIG-B; UBCH8
• NCBI Protein Information: ubiquitin/ISG15-conjugating enzyme E2 L6; ubiquitin-protein ligase L6; ubiquitin carrier protein L6; retinoic acid induced gene B protein; retinoic acid-induced gene B protein
• UniProt Protein Name: Ubiquitin/ISG15-conjugating enzyme E2 L6
• Protein Family: Ubiquitin/ISG15-conjugating enzyme
• UniProt Gene Name: UBE2L6
• UniProt Synonym Gene Names: UBCH8; RIG-B
• UniProt Entry Name: UB2L6_HUMAN

NCBI Description

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]

Uniprot Description

UBE2L6: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Promotes ubiquitination and subsequent proteasomal degradation of FLT3. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.19; Ubiquitin ligase; Ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 11q12

Cellular Component: cytosol

Molecular Function: protein binding; ISG15 conjugating enzyme activity; ubiquitin-protein ligase activity; ligase activity

Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; cytokine and chemokine mediated signaling pathway; protein modification process; protein ubiquitination; innate immune response; negative regulation of interferon type I production