Patched 1 (PTCH1) Recombinant Protein | PTCH1 recombinant protein

Recombinant Patched 1 (PTCH1)

Cat. Num. AAA2009672

• Gene Names: PTCH1; PTC; BCNS; HPE7; PTC1; PTCH; NBCCS; PTCH11
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Patched 1 (PTCH1); Recombinant Patched 1 (PTCH1); PTCH1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-LIIT PLDCFWEGAK LQSGTAYLLG KPPLRWTNFD PLEFLEELKK INYQVDSWEE MLNKAEVGHG YMDRPCLNPA DPDCPATAPN KNSTKPLDMA LVLNGGCHGL SRKYMHWQEE LIVGGTVKNS TGKLVSAHAL QTMFQLMTPK QMYEHFKGYE YVSHINWNED KAAAILEAWQ RTYVEVVHQS VAQNSTQKVL SFTTTTLDDI LKSFSD
• Sequence Length: 1447

• Applicable Applications for PTCH1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu227~Asp436 (Accession # Q13635) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 134254446
• NCBI GeneID: 5727
• NCBI Accession #: NP_000255.2
• NCBI GenBank Nucleotide #: NM_000264.3
• UniProt Accession #: Q13635; Q13463; Q5R1U7; Q5R1U9; Q5R1V0; Q5VZC0; Q5VZC2; Q86XG7; A3KBI9; E9PEJ8
• Molecular Weight: 27.4kDa
• NCBI Official Full Name: protein patched homolog 1 isoform L
• NCBI Official Synonym Full Names: patched 1
• NCBI Official Symbol: PTCH1
• NCBI Official Synonym Symbols: PTC; BCNS; HPE7; PTC1; PTCH; NBCCS; PTCH11
• NCBI Protein Information: protein patched homolog 1; PTCH protein +4'; PTCH protein -10; PTCH protein +12b; PTCH protein -3,4,5
• UniProt Protein Name: Protein patched homolog 1
• UniProt Gene Name: PTCH1
• UniProt Synonym Gene Names: PTCH; PTC; PTC1
• UniProt Entry Name: PTC1_HUMAN

NCBI Description

This gene encodes a member of the patched gene family. The encoded protein is the receptor for sonic hedgehog, a secreted molecule implicated in the formation of embryonic structures and in tumorigenesis, as well as the desert hedgehog and indian hedgehog proteins. This gene functions as a tumor suppressor. Mutations of this gene have been associated with basal cell nevus syndrome, esophageal squamous cell carcinoma, trichoepitheliomas, transitional cell carcinomas of the bladder, as well as holoprosencephaly. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences and biological validity cannot be determined currently. [provided by RefSeq, Jul 2008]

Uniprot Description

PTCH1: a multi-pass membrane protein member of the ?patched? family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein?s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin. In the embryo, found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud. Defects in PTCH1 are the cause of basal cell nevus syndrome (BCNS), also known as Gorlin syndrome. BCNS is an autosomal dominant disease characterized by nevoid basal cell carcinomas and developmental abnormalities such as rib and craniofacial alterations, polydactyly, syndactyly, and spina bifida. In addition, the patients suffer from a multitude of tumors like basal cell carcinomas, fibromas of the ovaries and heart, cysts of the skin, jaws and mesentery, as well as medulloblastomas and meningiomas. PTCH1 defects is also the cause of holoprosencephaly, the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres.

Protein type: Cell cycle regulation; Tumor suppressor; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: Golgi apparatus; intracellular membrane-bound organelle; perinuclear region of cytoplasm; postsynaptic density; plasma membrane; integral to membrane; caveola; midbody

Molecular Function: heparin binding; cyclin binding; protein binding; hedgehog receptor activity; cholesterol binding; protein complex binding; patched binding; smoothened binding

Biological Process: heart morphogenesis; hindlimb morphogenesis; epidermis development; regulation of mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; response to chlorate; response to estradiol stimulus; regulation of protein localization; negative regulation of osteoblast differentiation; embryonic limb morphogenesis; negative regulation of epithelial cell proliferation; response to drug; smoothened signaling pathway; response to retinoic acid; pharyngeal system development; negative regulation of multicellular organism growth; in utero embryonic development; negative regulation of transcription factor activity; neural tube patterning; keratinocyte proliferation; negative regulation of cell division; spinal cord motor neuron differentiation; limb morphogenesis; organ morphogenesis; dorsal/ventral pattern formation; ureteric bud branching; response to mechanical stimulus; neural plate pattern formation; negative regulation of smoothened signaling pathway; neural tube closure; embryonic organ development; protein processing; brain development; regulation of smoothened signaling pathway

Disease: Basal Cell Nevus Syndrome; Holoprosencephaly 7; Basal Cell Carcinoma, Susceptibility To, 1