Rabbit anti-Human, Mouse TBX6 Polyclonal Antibody | anti-TBX6 antibody

TBX6, ID (TBX6, T-box transcription factor TBX6) (AP)

Cat. Num. AAA6354249

• Gene Names: TBX6; SCDO5
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TBX6; Polyclonal Antibody; ID (TBX6; T-box transcription factor TBX6) (AP); T-box transcription factor TBX6; anti-TBX6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TBX6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-301 amino acids from the Central region of human TBX6.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TBX6 Antibody (Center) western blot analysis in ZR-75-1 cell line and mouse spleen tissue lysates (35ug/lane).This demonstrates the TBX6 antibody detected the TBX6 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.)

Related Product Information for anti-TBX6 antibody

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures.

Product Categories/Family for anti-TBX6 antibody

Antibodies; Transcription Factors

References

Fei, Q., et al. Spine 35(9):983-988(2010). Ghebranious, N., et al. J. Bone Miner. Res. 23(10):1576-1583(2008). Farin, H.F., et al. J. Biol. Chem. 282(35):25748-25759(2007). Papapetrou, C., et al. Genomics 55(2):238-241(1999). Yi, C.H., et al. Genomics 55(1):10-20(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 18201900
• NCBI GeneID: 6911
• NCBI Accession #: NP_004599.2
• NCBI GenBank Nucleotide #: NM_004608.3
• UniProt Accession #: O95947; Q8TAS4; Q9HA44
• Molecular Weight: 33,197 Da
• NCBI Official Full Name: T-box transcription factor TBX6
• NCBI Official Synonym Full Names: T-box 6
• NCBI Official Symbol: TBX6
• NCBI Official Synonym Symbols: SCDO5
• NCBI Protein Information: T-box transcription factor TBX6; T-box protein 6
• UniProt Protein Name: T-box transcription factor TBX6
• Protein Family: T-box transcription factor
• UniProt Gene Name: TBX6
• UniProt Synonym Gene Names: T-box protein 6
• UniProt Entry Name: TBX6_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Knockout studies in mice indicate that this gene is important for specification of paraxial mesoderm structures. [provided by RefSeq, Aug 2008]

Uniprot Description

TBX6: T-box transcription factor that plays an essential role in the determination of the fate of axial stem cells: neural vs mesodermal. Acts in part by down-regulating, a specific enhancer (N1) of SOX2, to inhibit neural development. Seems to play also an essential role in left/right axis determination and acts through effects on Notch signaling around the node as well as through an effect on the morphology and motility of the nodal cilia.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: nucleus

Molecular Function: DNA binding; transcription factor activity

Biological Process: negative regulation of neuron maturation; anatomical structure morphogenesis; transcription, DNA-dependent; mesoderm formation; positive regulation of transcription from RNA polymerase II promoter; cell fate specification; mesoderm development; negative regulation of transcription from RNA polymerase II promoter; somite rostral/caudal axis specification

Disease: Deafness, Autosomal Recessive 67