PSTPIP1 recombinant protein

PSTPIP1, 1-416aa, Human, His tag, E Coli

Cat. Num. AAA205025

• Gene Names: PSTPIP1; H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PSTPIP1; 1-416aa; Human; His tag; E Coli; Proline-serine-threonine phosphatase interacting protein 1; CD2BP1; CD2BP1L; CD2BP1S; H-PIP; PAPAS; PSTPIP; PSTPIP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMMPQLQF KDAFWCRDFT AHTGYEVLLQ RLLDGRKMCK DMEELLRQRA QAEERYGKEL VQIARKAGGQ TEINSLRASF DSLKQQMENV GSSHIQLALT LREELRSLEE FRERQKEQRK KYEAVMDRVQ KSKLSLYKKA MESKKTYEQK CRDADDAEQA FERISANGHQ KQVEKSQNKA RQCKDSATEA ERVYRQSIAQ LEKVRAEWEQ EHRTTCEAFQ LQEFDRLTIL RNALWVHSNQ LSMQCVKDDE LYEEVRLTLE GCSIDADIDS FIQAKSTGTE PPAPVPYQNY YDREVTPLTS SPGIQPSCGM IKRFSGLLHG SPKTTSLAAS AASTETLTPT PERNEGVYTA IAVQEIQGNP ASPAQEYRAL YDYTAQNPDE LDLSAGDILE VILEGEDGWW TVERNGQRGF VPGSYLEKL
• Sequence Length: 416

• Applicable Applications for PSTPIP1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PSTPIP1 recombinant protein

PSTPIP1 binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. Recombinant human PSTPIP1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PSTPIP1 recombinant protein

Signal Transduction

References

Shoham N.G., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:13501-13506; Wise C.A., et al. (2002) Hum. Mol. Genet. 11:961-969

NCBI and Uniprot Product Information

• NCBI GI #: 20149528
• NCBI GeneID: 9051
• NCBI Accession #: NP_003969
• NCBI GenBank Nucleotide #: NM_003978.4
• UniProt Accession #: O43586; O43585; O95657; B5BU74; B5BUK4
• Molecular Weight: 50.0kDa (439aa)
• NCBI Official Full Name: proline-serine-threonine phosphatase-interacting protein 1 isoform 1
• NCBI Official Synonym Full Names: proline-serine-threonine phosphatase interacting protein 1
• NCBI Official Symbol: PSTPIP1
• NCBI Official Synonym Symbols: H-PIP; PAPAS; CD2BP1; PSTPIP; CD2BP1L; CD2BP1S
• NCBI Protein Information: proline-serine-threonine phosphatase-interacting protein 1
• UniProt Protein Name: Proline-serine-threonine phosphatase-interacting protein 1
• Protein Family: Proline-serine-threonine phosphatase-interacting protein
• UniProt Gene Name: PSTPIP1
• UniProt Synonym Gene Names: CD2BP1; PEST phosphatase-interacting protein 1

NCBI Description

This gene encodes a cytoskeletal protein that is highly expressed in hemopoietic tissues. This protein functions via its interaction with several different proteins involved in cytoskeletal organization and inflammatory processes. It binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, downregulating CD2-triggered adhesion. It binds PEST-type protein tyrosine phosphatases (PTP) and directs them to c-Abl kinase to mediate c-Abl dephosphorylation, thereby, regulating c-Abl activity. It also interacts with pyrin, which is found in association with the cytoskeleton in myeloid/monocytic cells and modulates immunoregulatory functions. Mutations in this gene are associated with PAPA (pyogenic sterile arthritis, pyoderma gangrenosum, and acne) syndrome. It is hypothesized that the disease-causing mutations compromise physiologic signaling necessary for the maintenance of a proper inflammatory response. [provided by RefSeq, Mar 2016]

Uniprot Description

Involved in regulation of the actin cytoskeleton. May regulate WAS actin-bundling activity. Bridges the interaction between ABL1 and PTPN18 leading to ABL1 dephosphorylation. May play a role as a scaffold protein between PTPN12 and WAS and allow PTPN12 to dephosphorylate WAS. Has the potential to physically couple CD2 and CD2AP to WAS. Acts downstream of CD2 and CD2AP to recruit WAS to the T-cell:APC contact site so as to promote the actin polymerization required for synapse induction during T-cell activation (). Down-regulates CD2-stimulated adhesion through the coupling of PTPN12 to CD2. Also has a role in innate immunity and the inflammatory response. Recruited to inflammasomes by MEFV. Induces formation of pyroptosomes, large supramolecular structures composed of oligomerized PYCARD dimers which form prior to inflammatory apoptosis. Binding to MEFV allows MEFV to bind to PYCARD and facilitates pyroptosome formation. Regulates endocytosis and cell migration in neutrophils.