Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse SDHA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit anti-Mouse Succinate Dehydrogenase Complex Subunit A Polyclonal Antibody | anti-SDHA antibody
Succinate Dehydrogenase Complex Subunit A (SDHA) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse SDHA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse SDHA Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P. Samples: Mouse Tissue))
NCBI and Uniprot Product Information
Uniprot Description
SDHA: is the catalytic subunit of succinate dehydrogenase (SDH) complex II of the mitochondrial electron transport chain. Complex II contains four subunits: the flavoprotein catalytic subunit SDHA, iron-sulfur protein SDHB, and a cytochrome b560 composed of SDHC and SDHD. Interaction with SDH5 is required for FAD attachment. Responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Defects in SDHA cause defective mitochondrial oxidative phosphorylation, giving rise to heterogeneous clinical symptoms ranging from isolated organ dysfunction to multisystem disorder. Acetylation of SDHA may control entry of the substrate into the active site, thus regulating its enzymatic activity. Acetylated SDHA may be a SIRT3 substrate SIRT3 is a mitochondrial NAD(+)-dependent deacetylase. Increased succinate levels as a consequence of SDH deficiency inhibit hypoxia inducible factor-1alpha (HIF-1alpha) prolyl hydroxylases leading to sustained HIF-1alpha expression in tumours. Defects in SDHA cause of Leigh syndrome, a severe disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.
Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.3.5.1; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase; Tumor suppressor
Chromosomal Location of Human Ortholog: 13|13 C1
Cellular Component: membrane; mitochondrial inner membrane; mitochondrial respiratory chain complex II; mitochondrion; myelin sheath; nucleolus
Molecular Function: electron transfer activity; FAD binding; oxidoreductase activity; oxidoreductase activity, acting on the CH-CH group of donors; succinate dehydrogenase (ubiquinone) activity; succinate dehydrogenase activity
Biological Process: anaerobic respiration; mitochondrial electron transport, succinate to ubiquinone; nervous system development; succinate metabolic process; transport; tricarboxylic acid cycle
Research Articles on SDHA
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Product Notes
The SDHA sdha (Catalog #AAA2032306) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Succinate Dehydrogenase Complex Subunit A (SDHA) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Succinate Dehydrogenase Complex Subunit A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the SDHA sdha for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Succinate Dehydrogenase Complex Subunit A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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