IL17F recombinant protein

IL17F

Cat. Num. AAA517118

• Gene Names: IL17F; ML1; ML-1; CANDF6; IL-17F
• Applications: Cell Assay
• Synonyms: IL17F; Cytokine ML-1; IL17F recombinant protein

• Host: E Coli
• Form/Format: Lyophilized from a 0.2 um filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)

• Applicable Applications for IL17F recombinant protein: Cell Assay
• Species: Human
• Endotoxin Level: <1.0EU/ug of recombinant protein as determined by the LAL method
• Sample Cell Assay Data: The ED50 is 8.0-40.0ng/mL as determined by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells.
• Reconstitution: Reconstitute in sterile 4mM HCl at 25ug/mL (containing at least 0.1% human or bovine serum albumin)
• Preparation and Storage: The lyophilized protein is stable for at least one year from date of receipt at -70 degree C. Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree -8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

SDS-Page

(Purity: Recombinant Human IL17D resolved on a 15% SDS-PAGE gel under reducing conditions and stained with Coomassie Brilliant Blue G-250)

Related Product Information for IL17F recombinant protein

Recombinant Human IL17F was expressed in E Coli cells. The protein accession number is Q96PD4
Interleukin 17F is a disulfide-linked homodimeric protein belonging to the IL-17 family of proinflammatory cytokines. Members belonging to this family share conserved features of cysteine-knot structures but differ in their N-terminal regions. The T-helper cell produced IL-17 family is inducible by IL15 and IL23, and IL17F induces T-cell and PBMC activation and proliferation. It also performs cartilage matrix turnover and angiogenesis prevention. Out of the IL-17 family, IL17F is the most homologous to IL17 and dimerizes in a way similar to that of nerve growth factors (NGF) and other neurotrophins.

Product Categories/Family for IL17F recombinant protein

Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 16418375
• NCBI GeneID: 112744
• NCBI Accession #: NP_443104.1
• NCBI GenBank Nucleotide #: NM_052872.3
• UniProt Accession #: Q96PD4; Q6NSI0; Q7Z6P4; Q96PI8; Q9NUE6
• Molecular Weight: 18,045 Da
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: IL17F
• NCBI Official Synonym Symbols: ML1; ML-1; CANDF6; IL-17F
• NCBI Protein Information: interleukin-17F; cytokine ML-1
• UniProt Protein Name: Interleukin-17F
• Protein Family: Interleukin
• UniProt Gene Name: IL17F
• UniProt Synonym Gene Names: IL-17F
• UniProt Entry Name: IL17F_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. [provided by RefSeq, Jul 2008]

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: extracellular space; extracellular region

Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of transforming growth factor beta receptor signaling pathway; regulation of interleukin-8 biosynthetic process; lymphotoxin A biosynthetic process; cartilage development; cytokine biosynthetic process; regulation of interleukin-2 biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response

Disease: Candidiasis, Familial, 6