PSPH recombinant protein

PSPH Protein, Human, Recombinant

Cat. Num. AAA8121441

• Gene Names: PSPH; PSP; PSPHD
• Purity: >84% as determined by SDS-PAGE
• Synonyms: PSPH; PSPH Protein; Human; Recombinant; Human PSPH Protein; PSP Protein; PSPHD Protein; phosphoserine phosphatase; PSPH recombinant protein

• Host: E Coli
• Purity/Purification: >84% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Glu225

• Species: Human
• Predicted N Terminal: Met 1
• Tag: Native
• Protein Construction: A DNA sequence encoding the human PSpH(P78330)(Met 1-Glu 225) was expressed and purified.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for PSPH recombinant protein

Background: Phosphoserine phosphatase (PSPH) belongs to a subfamily of the phosphotransferases. PSPH is the rate-limiting enzyme in l-serine biosynthesis. It has previously been found that Phosphoserine phosphatase (PSPH) plays a role in epidermal homeostasis. Phosphoserine phosphatase (PSP) catalyzes the hydrolysis of phosphoserine to serine. Phosphoserine phosphatase (PSPH) expression has been examined in human-mouse somatic cell hybrids retaining different combination of human chromosomes. Phosphoserine phosphatase (PSPH) is expressed throughout the proliferative layer of the epidermis and hair follicles in rodent and human skin and is highly induced in SCC. In keratinocytes, Phosphoserine phosphatase (PSPH) is a cytoplasmic protein that primarily localizes to endosomes and is present primarily as a homodimer. Knock down of Phosphoserine phosphatase (PSPH) dramatically diminished SCC cell proliferation and cyclin D1 levels in the presence of exogenous of l-serine production suggesting a non-canonical role for Phosphoserine phosphatase (PSPH) in epithelial carcinogenesis. Phosphoserine phosphatase (PSPH) is highly induced in proliferative normal keratinocytes and skin tumors. Phosphoserine phosphatase (PSPH) appears to be critical for the proliferation of SCC cells; however, this phenomenon may not involve the phosphoserine metabolic pathway.

References

Bachelor MA, et al. (2011) L-3-Phosphoserine phosphatase (PSPH) regulates cutaneous squamous cell carcinoma proliferation independent of L-serine biosynthesis. J Dermatol Sci. 63(3): 164-72.Koch GA, et al. (1983) Assignment of the human phosphoserine phosphatase gene (PSP) to the pter leads to q22 region of chromosome 7. Cytogenet Cell Genet. 35(1): 67-9.Jaeken J, et al. (1997) Phosphoserine phosphatase deficiency in a patient with Williams syndrome. J Med Genet. 34(7): 594-6.

NCBI and Uniprot Product Information

• NCBI GI #: 46249388
• NCBI GeneID: 5723
• NCBI Accession #: NP_004568.2
• NCBI GenBank Nucleotide #: NM_004577.3
• UniProt Accession #: P78330; Q7Z3S5; B2RCR5
• Molecular Weight: 25,008 Da
• NCBI Official Full Name: phosphoserine phosphatase
• NCBI Official Synonym Full Names: phosphoserine phosphatase
• NCBI Official Symbol: PSPH
• NCBI Official Synonym Symbols: PSP; PSPHD
• NCBI Protein Information: phosphoserine phosphatase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; PSPase
• UniProt Protein Name: Phosphoserine phosphatase
• UniProt Gene Name: PSPH
• UniProt Synonym Gene Names: PSP; PSPase
• UniProt Entry Name: SERB_HUMAN

NCBI Description

The protein encoded by this gene belongs to a subfamily of the phosphotransferases. This encoded enzyme is responsible for the third and last step in L-serine formation. It catalyzes magnesium-dependent hydrolysis of L-phosphoserine and is also involved in an exchange reaction between L-serine and L-phosphoserine. Deficiency of this protein is thought to be linked to Williams syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

PSPH: Catalyzes the last step in the biosynthesis of serine from carbohydrates. The reaction mechanism proceeds via the formation of a phosphoryl-enzyme intermediates. Defects in PSPH are the cause of phosphoserine phosphatase deficiency (PSPHD)[MIM:614023]. A disorder that results in pre- and postnatal growth retardation, moderate psychomotor retardation and facial features suggestive of Williams syndrome. Belongs to the SerB family.

Protein type: EC 3.1.3.3; Motility/polarity/chemotaxis; Hydrolase; Amino Acid Metabolism - glycine, serine and threonine

Chromosomal Location of Human Ortholog: 7p11.2

Cellular Component: neuron projection; cytoplasm; cytosol

Molecular Function: protein homodimerization activity; phosphoserine phosphatase activity; magnesium ion binding; calcium ion binding

Biological Process: response to nutrient levels; L-serine biosynthetic process; dephosphorylation; L-serine metabolic process; response to mechanical stimulus; response to testosterone stimulus; amino acid biosynthetic process

Disease: Phosphoserine Phosphatase Deficiency