Proteasome 26S Subunit Recombinant Protein | PSMD9 recombinant protein

Recombinant Human Proteasome 26S Subunit, Non-ATPase 9

Cat. Num. AAA146072

• Gene Names: PSMD9; p27; Rpn4
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Proteasome 26S Subunit; Recombinant Human Proteasome 26S Subunit; Non-ATPase 9; PSMD9 Human; Non-ATPase 9 Human Recombinant; p27; Rpn4; 26S proteasome non-ATPase regulatory subunit 9; 26S proteasome regulatory subunit p27; PSMD9; PSMD9 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The PSMD9 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 10% glycerol and 1mM DTT.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR
• Sequence Length: 118

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks.Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PSMD9 recombinant protein

Description: PSMD9 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 246 amino acids (1-223) and having a molecular mass of 27.1kDa. PSMD9 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: PSMD9 is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. PSMD9 acts as a chaperone through the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex compilation, PSMD9 is part of an intermediate PSMD9:PSMC6:PSMC3 module, also called modulator trimer complex. PSMD9 is released during the further base assembly process.

Product Categories/Family for PSMD9 recombinant protein

Enzymes; Proteasome

NCBI and Uniprot Product Information

• NCBI GI #: 386869374
• NCBI GeneID: 5715
• NCBI Accession #: NP_001248329.1
• NCBI GenBank Nucleotide #: NM_001261400.2
• UniProt Accession #: O00233; Q9BQ42; B2RD35; G3V1Q6
• Molecular Weight: 13,053 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 9 isoform 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 9
• NCBI Official Symbol: PSMD9
• NCBI Official Synonym Symbols: p27; Rpn4
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 9; 26S proteasome regulatory subunit p27; homolog of rat Bridge 1; proteasome 26S non-ATPase regulatory subunit 9
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 9
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD9
• UniProt Entry Name: PSMD9_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

PSMD9: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Belongs to the proteasome subunit p27 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Protease; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12q24.31-q24.32

Cellular Component: nucleoplasm; cytoplasm; proteasome regulatory particle; cytosol; nucleus

Molecular Function: protein binding; transcription coactivator activity; bHLH transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; negative regulation of insulin secretion; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis