Rabbit PSMD9 Polyclonal Antibody | anti-PSMD9 antibody

PSMD9 Polyclonal Antibody

Cat. Num. AAA3011274

• Gene Names: PSMD9; p27; Rpn4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PSMD9; Polyclonal Antibody; PSMD9 Polyclonal Antibody; 26S proteasome non-ATPase regulatory subunit 9; 26S proteasome regulatory subunit p27; Homolog of rat Bridge 1; p27; Proteasome (prosome macropain) 26S subunit non ATPase 9; Proteasome 26S non ATPase regulatory subunit 9; Proteasome 26S subunit non ATPase 9; Psmd9; PSMD9_HUMAN; Rpn4; anti-PSMD9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PSMD9 polyclonal antibody detects endogenous levels of PSMD9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 223

• Applicable Applications for anti-PSMD9 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human PSMD9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PSMD9 polyclonal antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analysis of PSMD9 polyclonal antibody in paraffin-embedded Mouse Kidney tissue.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Hela cells, using PSMD9 polyclonal antibody)

Related Product Information for anti-PSMD9 antibody

Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9: PSMC6: PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process.

NCBI and Uniprot Product Information

• NCBI GI #: 317373521
• NCBI GeneID: 5715
• UniProt Accession #: O00233
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 9
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 9
• NCBI Official Symbol: PSMD9
• NCBI Official Synonym Symbols: p27; Rpn4
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 9
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 9
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD9
• UniProt Entry Name: PSMD9_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Uniprot Description

PSMD9: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Belongs to the proteasome subunit p27 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Proteasome complex; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 12q24.31-q24.32

Cellular Component: nucleoplasm; proteasome regulatory particle; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; transcription coactivator activity; bHLH transcription factor binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; positive regulation of transcription, DNA-dependent; positive regulation of insulin secretion; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; negative regulation of insulin secretion; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis