Goat TRAF2 Polyclonal Antibody | anti-TRAF2 antibody

Goat anti-TRAF2 Antibody

Cat. Num. AAA421174

• Gene Names: TRAF2; TRAP; TRAP3; MGC:45012
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TRAF2; Polyclonal Antibody; Goat anti-TRAF2 Antibody; TNF receptor-associated factor 2; MGC: 45012; TRAP; TRAP3; OTTHUMP00000064745; tumor necrosis factor type 2 receptor associated protein 3; TRAF2 antibody; TNF receptor-associated factor 2 antibody; MGC: 45012 antibody; TRAP antibody; TRAP3 antibody; OTTHUMP00000064745 antibody; tumor necrosis factor type 2 receptor associated protein 3 antibody; anti-TRAF2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: KMEAKNSYVRDD
• Sequence Length: 501

• Applicable Applications for anti-TRAF2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 128000.; Immunohistochemistry: In paraffin embedded Human Kidney shows cytoplasm and select nuclear staining. Recommended concentration, 3-6ug/ml.; Western Blot: Approx 50kDa band observed in Human Ovary lysates (calculated MW of 55.9kDa according to NP_066961.2). In transfected HEK293 transiently expressing Human TRAF2 a band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 48kDa protein is observed in both. Recommended concentration: 0.1-0.3ug/ml.
• Immunogen: Peptide with sequence C-KMEAKNSYVRDD, from the C Terminus of the protein sequence according to NP_066961.2.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.1ug/ml) staining of human ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB)

(HEK293 overexpressing Human TRAF2 (RC208110) and probed (mock transfection in first lane), tested by Origene.)

Immunohistochemistry (IHC)

((3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 22027612
• NCBI GeneID: 7186
• NCBI Accession #: NP_066961.2
• NCBI GenBank Nucleotide #: NM_021138.3
• Molecular Weight: 53,055 Da
• NCBI Official Full Name: TNF receptor-associated factor 2
• NCBI Official Synonym Full Names: TNF receptor associated factor 2
• NCBI Official Symbol: TRAF2
• NCBI Official Synonym Symbols: TRAP; TRAP3; MGC:45012
• NCBI Protein Information: TNF receptor-associated factor 2
• UniProt Protein Name: TNF receptor-associated factor 2
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF2
• UniProt Synonym Gene Names: TRAP3
• UniProt Entry Name: TRAF2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF2: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'- linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin- protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation; this does not depend on the TRAF2 RING-type zinc finger domain. Homotrimer, and heterotrimer with TRAF1 and TRAF3 (via TRAF domain). The domain containing the RING-type and the first TRAF-type zinc finger can also form homodimers (in vitro). Interacts with TNFRSF1B/TNFR2, TNFRSF4, TNFRSF5/CD40, CD27/TNFRSF7, TNFRSF8/CD30, TNFRSF9/CD137, TNFRSF11A/RANK, TNFRSF13B/TACI, TNFRSF14, TNFRSF16/NGFR, TNFRSF17/BCMA, TNFRSF18/AITR, TNFRSF19/TROY, TNFRSF19L/RELT, XEDAR, EDAR, Epstein-Barr virus BNFL1/LMP-1 and IL15RA. Interacts with CDK9, CSK, MAP3K1, MAP3K5, MAP3K11, MAP3K14, MAP4K2, RIPK1, RIPK2, TNIK, TBK1, SPHK1, TRADD, TRAFD1, TRAIP, TANK/ITRAF, TNFAIP3, TDP2, MAVS/IPS1, TICAM1 and TRPC4AP. Interacts with CASP8AP2, NFATC2IP, PEG3 and HIVEP3. Interacts with BIRC2 and BIRC3 N- terminus; a single BIRC2 or BIRC3 molecule interacts with a heterotrimer formed by TRAF1 and TRAF2, or a TRAF2 homotrimer. Identified in a complex composed of TRAF2, TRAF3, BIRC2 and BIRC3. Interaction with BIRC2 and/or BIRC3 is essential for degradation of NFKBIA and activation of NF-kappa-B. Interacts with CYLD, USP48, DAB2IP, IKKA and IKKB. Identified in a complex with TNFRSF1A, RIPK1 and IKKB. Interacts (via 'Lys-63'-linked polyubiquitin chains) with TAB2 and TAB3. Interacts with ERN1 and TAOK3. Interaction with TAOK3 is facilitated under ER stress conditions, such as treatment with tunicamycin, and may promote TRAF2 phosphorylation. Has very low E3 ubiquitin ligase activity in the absence of sphingosine-1-phosphate. E3 ubiquitin ligase activity is strongly activated by cytoplasmic sphingosine-1- phosphate. Belongs to the TNF receptor-associated factor family. A subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Ligase; Autophagy; Ubiquitin ligase; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cell cortex; cytoplasm; cytosol; internal side of plasma membrane; lipid raft; ubiquitin ligase complex; vesicle membrane

Molecular Function: CD40 receptor binding; enzyme binding; identical protein binding; ligase activity; mitogen-activated protein kinase kinase kinase binding; protein binding; protein complex binding; protein kinase binding; protein phosphatase binding; signal transducer activity; sphingolipid binding; thioesterase binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: activation of NF-kappaB transcription factor; activation of NF-kappaB-inducing kinase; caspase activation; cellular protein complex assembly; I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-2 production; positive regulation of JNK activity; positive regulation of T cell activation; positive regulation of T cell cytokine production; positive regulation of transcription factor activity; protein autoubiquitination; protein catabolic process; protein complex assembly; protein heterooligomerization; regulation of apoptosis; regulation of immunoglobulin secretion; signal transduction; tumor necrosis factor-mediated signaling pathway