Canine Prostate specific antigen ELISA Kit | PSA elisa kit

Canine Prostate specific antigen ELISA Kit

Cat. Num. AAA737089

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Canine
• Synonyms: Prostate specific antigen; Canine Prostate specific antigen ELISA Kit; PSA elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This PSA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PSA. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSA elisa kit

Principle of the Assay: PSA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PSA antibody and an PSA-HRP conjugate. The assay sample and buffer are incubated together with PSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PSA concentration since PSA from samples and PSA-HRP conjugate compete for the anti-PSA antibody binding site. Since the number of sites is limited, as more sites are occupied by PSA from the sample, fewer sites are left to bind PSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PSA concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PSA elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 4502173
• NCBI GeneID: 354
• NCBI Accession #: NP_001639.1
• NCBI GenBank Nucleotide #: NM_001648.2
• Molecular Weight: 25,116 Da
• NCBI Official Full Name: prostate-specific antigen isoform 1 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen; seminin; P-30 antigen; kallikrein-3; semenogelase; gamma-seminoprotein; prostate specific antigen
• UniProt Protein Name: Prostate-specific antigen
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin
• UniProt Entry Name: KLK3_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.77

Chromosomal Location of Human Ortholog: 19q13.41

Cellular Component: extracellular region; nucleus

Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis