Canine Presenilin 1 (PS-1) ELISA Kit | PS1 elisa kit

Canine Presenilin 1 (PS-1) ELISA Kit

Cat. Num. AAA7221260

• Gene Names: PSEN1; AD3; FAD; PS1; PS-1; S182
• Reactivity: Canine
• Synonyms: Presenilin 1 (PS-1); Canine Presenilin 1 (PS-1) ELISA Kit; PS1 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of PS-1. No significant cross-reactivity or interference between PS-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PS-1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PS1 elisa kit

Intended Uses: This PS-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PS-1. This ELISA kit for research use only!

Principle of the Assay: PS-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PS-1 antibody and an PS-1-HRP conjugate. The assay sample and buffer are incubated together with PS-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PS-1 concentration since PS-1 from samples and PS-1-HRP conjugate compete for the anti-PS-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PS-1 from the sample, fewer sites are left to bind PS-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PS-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PS1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 1709856
• NCBI GeneID: 5663
• Molecular Weight: 48,997 Da
• NCBI Official Full Name: Presenilin-1
• NCBI Official Synonym Full Names: presenilin 1
• NCBI Official Symbol: PSEN1
• NCBI Official Synonym Symbols: AD3; FAD; PS1; PS-1; S182
• NCBI Protein Information: presenilin-1
• UniProt Protein Name: Presenilin-1
• Protein Family: FHA domain-containing protein
• UniProt Gene Name: PSEN1
• UniProt Synonym Gene Names: AD3; PS1; PSNL1; PS-1; PS1-CTF12
• UniProt Entry Name: PSN1_HUMAN

NCBI Description

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq, Aug 2008]

Uniprot Description

PSEN1: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Regulates epithelial- cadherin function. Five alternative splice isoforms have been identified.

Protein type: Cell surface; Protease; Mitochondrial; Membrane protein, integral; Membrane protein, multi-pass; EC 3.4.23.-

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: Golgi apparatus; nuclear outer membrane; centrosome; cell surface; smooth endoplasmic reticulum; mitochondrion; lysosomal membrane; integral to plasma membrane; integral to membrane; lipid raft; ciliary rootlet; kinetochore; cell soma; membrane; perinuclear region of cytoplasm; mitochondrial inner membrane; apical plasma membrane; cytoplasmic vesicle; dendritic shaft; neuromuscular junction; endoplasmic reticulum membrane; nuclear membrane; rough endoplasmic reticulum; endoplasmic reticulum; cell cortex; Z disc; Golgi membrane; growth cone; axon

Molecular Function: protein binding; cadherin binding; calcium channel activity; endopeptidase activity; beta-catenin binding; aspartic-type endopeptidase activity; PDZ domain binding

Biological Process: positive regulation of catalytic activity; extracellular matrix organization and biogenesis; activation of MAPKK activity; positive regulation of apoptosis; beta-amyloid formation; regulation of synaptic plasticity; positive regulation of coagulation; Wnt receptor signaling pathway through beta-catenin; choline transport; T cell receptor signaling pathway; T cell activation during immune response; post-embryonic development; mitochondrial transport; extracellular matrix disassembly; positive regulation of MAP kinase activity; epithelial cell proliferation; cell-cell adhesion; negative regulation of axonogenesis; negative regulation of neuron apoptosis; embryonic limb morphogenesis; autophagic vacuole formation; somitogenesis; skin morphogenesis; regulation of phosphorylation; Notch receptor processing; negative regulation of ubiquitin-protein ligase activity; neuron development; Cajal-Retzius cell differentiation; hemopoietic progenitor cell differentiation; response to oxidative stress; negative regulation of apoptosis; regulation of synaptic transmission, glutamatergic; protein maturation; neuron migration; protein amino acid glycosylation; cell fate specification; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; protein transport; amyloid precursor protein catabolic process; beta-amyloid metabolic process; regulation of resting membrane potential; brain morphogenesis; heart looping; positive regulation of receptor recycling; dorsoventral neural tube patterning; blood vessel development; Notch signaling pathway; L-glutamate transport; thymus development; membrane protein ectodomain proteolysis; memory; negative regulation of epidermal growth factor receptor activity; smooth endoplasmic reticulum calcium ion homeostasis; endoplasmic reticulum calcium ion homeostasis; neuron apoptosis; cerebral cortex cell migration; skeletal morphogenesis; regulation of protein binding; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein processing; synaptic vesicle targeting; response to DNA damage stimulus

Disease: Pick Disease Of Brain; Alzheimer Disease 3; Frontotemporal Dementia; Acne Inversa, Familial, 3; Cardiomyopathy, Dilated, 1u