Dot Blot (DB)
(Dot blot analysis of rat TSC1 Antibody (Phospho Y297) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit anti-Rat TSC1 Polyclonal Antibody | anti-TSC1 antibody
TSC1, Phosphorylated (Y297) (Tsc1, Hamartin, Tuberous sclerosis 1 protein homolog) (Biotin)
Reactivity
Rat
Applications
ELISA, Dot Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TSC1; Polyclonal Antibody; Phosphorylated (Y297) (Tsc1; Hamartin; Tuberous sclerosis 1 protein homolog) (Biotin); Tsc1; Tuberous sclerosis 1 protein homolog; anti-TSC1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-TSC1 antibody
ELISA (EIA), Dot Blot (DB)
Application Notes
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Rat TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y297 of rat TSC1.
Conjugate
Biotin
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of rat TSC1 Antibody (Phospho Y297) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Dot Blot (DB)
(Dot blot analysis of rat TSC1 Antibody (Phospho Y297) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Related Product Information for anti-TSC1 antibody
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity).
Product Categories/Family for anti-TSC1 antibody
References
Inoue, H., et al. Biosci. Biotechnol. Biochem. 73(11):2488-2493(2009). Di Nardo, A., et al. J. Neurosci. 29(18):5926-5937(2009). Chen, P., et al. Exp. Mol. Pathol. 86(2):101-107(2009). Momose, S., et al. Biochem. Biophys. Res. Commun. 356(3):693-698(2007). Goncharova, E., et al. J. Cell Biol. 167(6):1171-1182(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
129,022 Da
NCBI Official Full Name
hamartin
NCBI Official Synonym Full Names
tuberous sclerosis 1
NCBI Official Symbol
Tsc1
NCBI Protein Information
hamartin; tuberous sclerosis 1 protein homolog
UniProt Protein Name
Hamartin
Protein Family
UniProt Gene Name
Tsc1
UniProt Entry Name
TSC1_RAT
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Product Notes
The TSC1 tsc1 (Catalog #AAA6359190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSC1, Phosphorylated (Y297) (Tsc1, Hamartin, Tuberous sclerosis 1 protein homolog) (Biotin) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TSC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Dot Blot (DB). ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TSC1 tsc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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