Typical Testing Data/Standard Curve (for reference only)
Canine P selectin ELISA Kit | PS elisa kit
Canine P selectin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: SELP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SELP antibody and an SELP-HRP conjugate. The assay sample and buffer are incubated together with SELP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SELP concentration since SELP from samples and SELP-HRP conjugate compete for the anti-SELP antibody binding site. Since the number of sites is limited, as more sites are occupied by SELP from the sample, fewer sites are left to bind SELP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SELP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
Uniprot Description
SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 1q22-q25
Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane
Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycosphingolipid binding; glycoprotein binding; calcium-dependent protein binding; sialic acid binding; fucose binding
Biological Process: platelet activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; positive regulation of phosphoinositide 3-kinase cascade; heterophilic cell adhesion; leukocyte adhesion; platelet degranulation; regulation of integrin activation; cell adhesion; blood coagulation; inflammatory response; leukocyte tethering or rolling; leukocyte migration
Disease: Ige Responsiveness, Atopic
Research Articles on PS
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Product Notes
The Canine PS selp (Catalog #AAA741062) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA741062 ELISA Kit recognizes Canine PS. It is sometimes possible for the material contained within the vial of "P selectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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