Typical Testing Data/Standard Curve (for reference only)
P-Selectin ELISA Kit | SELP elisa kit
P-Selectin ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The PSA ELISA test is a solid phase assay based on a streptavidin-biotin principle. The standards, samples and a reagent mixture of anti-PSA enzyme and biotin conjugates (conjugate reagent) are added into the wells that are coated with streptavidin. PSA in the serum forms a sandwich between two highly specific anti-PSA antibodies, labeled with biotin and HRP. Simultaneously, the biotinylated antibody is immobilized onto the wells through a high affinity streptavidin-biotin interaction. Unbound protein and excess biotin/enzyme conjugated reagent are washed off by washing buffer. Upon addition of the substrate, the intensity of the color developed is directly proportional to the concentration of PSA in the samples. A standard curve is prepared relating color intensity to the concentration of the PSA.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]
Uniprot Description
SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 1q22-q25
Cellular Component: cytoplasm; external side of plasma membrane; extracellular space; integral to plasma membrane; nucleoplasm; plasma membrane; platelet alpha granule membrane; platelet dense granule membrane
Molecular Function: calcium-dependent protein binding; fucose binding; glycoprotein binding; glycosphingolipid binding; heparin binding; lipopolysaccharide binding; protein binding; sialic acid binding
Biological Process: blood coagulation; cell adhesion; defense response to Gram-negative bacterium; heterophilic cell adhesion; inflammatory response; leukocyte adhesion; leukocyte migration; leukocyte tethering or rolling; platelet activation; platelet degranulation; positive regulation of cell adhesion; positive regulation of leukocyte migration; positive regulation of phosphoinositide 3-kinase cascade; regulation of cellular extravasation; regulation of integrin activation; response to lipopolysaccharide; response to organic cyclic substance
Disease: Ige Responsiveness, Atopic
Research Articles on SELP
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Product Notes
The SELP selp (Catalog #AAA495351) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "P-Selectin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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