Enteropeptidase/Enterokinase Assay Kit | TMPRSS15 assay kit

Enteropeptidase / Enterokinase Activity Fluorometric Assay Kit

Cat. Num. AAA841586

• Gene Names: TMPRSS15; ENTK; PRSS7
• Synonyms: Enteropeptidase/Enterokinase; Enteropeptidase / Enterokinase Activity Fluorometric Assay Kit; TMPRSS15 assay kit

• Specificity: The Kit for Entamoeba histolytica (E.histolytica) genomes is designed for the in vitro quantification of E.histolytica genomes. The kit is designed to have a broad detection profile. Specifically, the primers represent 100% homology with over 95% of the NCBI database reference sequences available at the time of design. The dynamics of genetic variation means that new sequence information may become available after the initial design. Periodically reviews the detection profiles of our kits and when required releases new versions.
• Sequence Length: 1019

• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TMPRSS15 assay kit

Background/Introduction: Entamoeba histolytica is an anaerobic, protozoan, intestinal parasite responsible for a disease called amoebiasis. It usually occurs in the large intestine and causes internal inflammation. It belongs to the genus Entamoeba and class Archamoeba. Amongst parasitic diseases, E. histolytica is one of the leading causes of morbidity and mortality in developing countries. E. histolytica is transmitted by ingestion of exit body containing cysts from faecally contaminated food and water or from hands. Due to their protective walls, the cysts can remain viable for several weeks in external environments. Species within this genus are small, single celled organisms with an anterior bulge representing a lobose pseudopod. The E. histolytica trophozoites are oblong and approximately 15-20uM in length, whereas the cysts are spherical and typically 12-15 uM in diameter. Entamoeba cysts are most commonly transmitted by ingestion so must be extremely robust to survive the hostile environment of the stomach. The cysts transform to trophozoites in the small intestine where they multiply by binary fission to then colonise the large intestine. They cause major calcium ion influx to the cells of the large intestine resulting in cell death and ulcer formation. The Trophozoites subsequently form new cysts which are excreted once more in faeces. Infection with E. histolytica generally causes mild symptoms such as abdominal pain, flatulence and diarrhoea, but more severe infections can lead to amoebosis. This is a condition encompassing amoebic dysentery characterized by severe abdominal pain, fever and blood in the faeces and less commonly amoebic liver abscesses.

Product Categories/Family for TMPRSS15 assay kit

Metabolism Assays; Amino Acids & Proteins; Metabolism Assays; Metabolism Assays (A-Z); Metabolism Assays; Other Metabolism-Related Assays

NCBI and Uniprot Product Information

• NCBI GI #: 223942069
• NCBI GeneID: 5651
• NCBI Accession #: NP_002763.2
• NCBI GenBank Nucleotide #: NM_002772.2
• Molecular Weight: 112,935 Da
• NCBI Official Full Name: enteropeptidase
• NCBI Official Synonym Full Names: transmembrane protease, serine 15
• NCBI Official Symbol: TMPRSS15
• NCBI Official Synonym Symbols: ENTK; PRSS7
• NCBI Protein Information: enteropeptidase
• UniProt Protein Name: Enteropeptidase
• Protein Family: Enteropeptidase
• UniProt Gene Name: TMPRSS15
• UniProt Synonym Gene Names: ENTK; PRSS7
• UniProt Entry Name: ENTK_HUMAN

NCBI Description

This gene encodes an enzyme that converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive. [provided by RefSeq, Jul 2008]

Uniprot Description

PRSS7: Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases. Defects in TMPRSS15 are a cause of enterokinase deficiency (ENTKD); a life-threatening intestinal malabsorption disorder characterized by diarrhea and failure to thrive. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.9; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: integral to membrane; brush border

Molecular Function: serine-type endopeptidase activity; scavenger receptor activity

Biological Process: receptor-mediated endocytosis; proteolysis

Disease: Enterokinase Deficiency