Protease, Serine 1 (PRSS1) Recombinant Protein | PRSS1 recombinant protein

Recombinant Protease, Serine 1 (PRSS1)

Cat. Num. AAA2105253

• Gene Names: PRSS1; TRP1; TRY1; TRY4; TRYP1
• Applications: SDS-Page, Western Blot
• Purity: > 95%
• Synonyms: Protease; Serine 1 (PRSS1); Recombinant Protease; TRP1; TRY1; TRY4; TRYP1; Trypsin 1; Cationic Trypsinogen; Beta-trypsin; Serine protease 1; Trypsin I; PRSS1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic Expression
• Purity/Purification: > 95%
• Form/Format: Freeze-Dried Powder; PBS, pH7.4, Containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Ile24~Ser247
• Sequence Length: 247

• Applicable Applications for PRSS1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Species: Human
• Tag: N-terminal His-Tag
• Isoelectric Point: 7.6
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Subcellular Location: Secreted, Extracellular Matrix
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for PRSS1 recombinant protein

Enzyme & Kinase; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 136408
• NCBI GeneID: 5644
• UniProt Accession #: P07477
• Molecular Weight: Predicted MW: 27.8kDa; Accurate MW: 30kDa
• NCBI Official Full Name: Trypsin-1
• NCBI Official Synonym Full Names: serine protease 1
• NCBI Official Symbol: PRSS1
• NCBI Official Synonym Symbols: TRP1; TRY1; TRY4; TRYP1
• NCBI Protein Information: trypsin-1
• UniProt Protein Name: Trypsin-1
• Protein Family: Anionic trypsin
• UniProt Gene Name: PRSS1
• UniProt Synonym Gene Names: TRP1; TRY1; TRYP1
• UniProt Entry Name: TRY1_HUMAN

NCBI Description

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]

Uniprot Description

trypsin 1: Has activity against the synthetic substrates Boc-Phe- Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val- Pro-Arg-Mec. The single-chain form is more active than the two- chain form against all of these substrates. Defects in PRSS1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Belongs to the peptidase S1 family.

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.4

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular region

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; vitamin metabolic process; cobalamin metabolic process; digestion; proteolysis; water-soluble vitamin metabolic process

Disease: Trypsinogen Deficiency; Pancreatitis, Hereditary