Rat Anionic trypsin-1 ELISA Kit | Prss1 elisa kit

Rat Anionic trypsin-1 ELISA Kit

Cat. Num. AAA761066

• Gene Names: Prss1; Try1; PTRYI; RATPTRYI
• Reactivity: Rat
• Synonyms: Anionic trypsin-1; Rat Anionic trypsin-1 ELISA Kit; Prss1/Anionic trypsin-1/Serine protease 1/Anionic trypsin I/Pretrypsinogen I/; Prss1 elisa kit

• Reactivity: Rat
• Sequence Length: 246

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.625-40ng/ml
• Sensitivity: 0.375ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Prss1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Prss1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Prss1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Prss1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Prss1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6981420
• NCBI GeneID: 24691
• NCBI Accession #: NP_036767.1
• NCBI GenBank Nucleotide #: NM_012635.1
• Molecular Weight: 25,959 Da
• NCBI Official Full Name: anionic trypsin-1
• NCBI Official Synonym Full Names: protease, serine 1
• NCBI Official Symbol: Prss1
• NCBI Official Synonym Symbols: Try1; PTRYI; RATPTRYI
• NCBI Protein Information: anionic trypsin-1
• UniProt Protein Name: Anionic trypsin-1
• Protein Family: Anionic trypsin
• UniProt Gene Name: Prss1
• UniProt Synonym Gene Names: Try1
• UniProt Entry Name: TRY1_RAT

NCBI Description

catalyzes the cleavage of Arg-Xaa, Lys-Xaa [RGD, Feb 2006]

Uniprot Description

trypsin 1: Has activity against the synthetic substrates Boc-Phe- Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val- Pro-Arg-Mec. The single-chain form is more active than the two- chain form against all of these substrates. Defects in PRSS1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.4; Secreted, signal peptide; Secreted; Protease

Cellular Component: extracellular space

Molecular Function: metal ion binding; serine-type endopeptidase activity

Biological Process: digestion; proteolysis; response to caffeine; response to nicotine; response to nutrient; response to organic substance