Rabbit anti-Human, Mouse CDKN1A Polyclonal Antibody | anti-CDKN1A antibody

CDKN1A, CT (CDKN1A, CAP20, CDKN1, CIP1, MDA6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, Melanoma differentiation-associated protein 6, p21) (APC)

Cat. Num. AAA6284601

• Gene Names: CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CDKN1A; Polyclonal Antibody; CT (CDKN1A; CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein 6; p21) (APC); p21; anti-CDKN1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-CDKN1A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 116-146 in the C-terminal region of human CDKN1A, UniProt Accession #NP_000380.1; NP_510867.1;P38936.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug) usingMBS6001177. This demonstrates that MBS6001177 detected the CDKN1A protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (35ug) usingMBS6001177. This demonstrates that MBS6001177 detected the CDKN1A protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma using MBS6001177 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6001177 for immunohistochemistry.)

Immunofluorescence (IF)

(Fluorescent image of A549 cell stained withMBS6001177. A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MBS6001177 (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C).CDKN1A immunoreactivity is localized to Nucleus significantly.)

Related Product Information for anti-CDKN1A antibody

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or-CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene.

Product Categories/Family for anti-CDKN1A antibody

Antibodies; Protein Kinase Inhibitors

References

Hu, F., et al. Oncogene 29(40):5464-5474(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Jiang, P., et al. Acta Biochim. Biophys. Sin. (Shanghai) 42(9):671-676(2010). Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010). In press: Do Nascimento Borges, B., et al. In Vivo 24(4):579-582(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 11386203
• NCBI GeneID: 1026
• NCBI Accession #: NP_000380.1; NP_510867.1
• NCBI GenBank Nucleotide #: NM_000389.4
• UniProt Accession #: P38936; Q14010; Q9BUT4
• Molecular Weight: 18,119 Da
• NCBI Official Full Name: cyclin-dependent kinase inhibitor 1
• NCBI Official Synonym Full Names: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• NCBI Official Symbol: CDKN1A
• NCBI Official Synonym Symbols: P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1
• NCBI Protein Information: cyclin-dependent kinase inhibitor 1; DNA synthesis inhibitor; CDK-interacting protein 1; CDK-interaction protein 1; wild-type p53-activated fragment 1; melanoma differentiation associated protein 6
• UniProt Protein Name: Cyclin-dependent kinase inhibitor 1
• UniProt Gene Name: CDKN1A
• UniProt Synonym Gene Names: CAP20; CDKN1; CIP1; MDA6; PIC1; SDI1; WAF1; MDA-6
• UniProt Entry Name: CDN1A_HUMAN

NCBI Description

This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Multiple alternatively spliced variants have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

p21Cip1: a cell-cycle regulatory protein that Interacts with cyclin-CDK2 and -CDK4, inhibiting cell cycle progression at G1. Its expression is tightly controlled by p53, through which this protein mediates the p53-dependent cell cycle arrest at G1 phase.

Protein type: Inhibitor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 6p21.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol

Molecular Function: cyclin-dependent protein kinase inhibitor activity; cyclin binding; protein binding; cyclin-dependent protein kinase activating kinase activity; metal ion binding; ubiquitin protein ligase binding; protein complex binding

Biological Process: nerve growth factor receptor signaling pathway; response to arsenic; response to toxin; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; protein amino acid phosphorylation; negative regulation of cell proliferation; positive regulation of fibroblast proliferation; positive regulation of B cell proliferation; G2/M transition of mitotic cell cycle; cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; response to corticosterone stimulus; response to X-ray; response to drug; epidermal growth factor receptor signaling pathway; organ regeneration; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; negative regulation of cyclin-dependent protein kinase activity; response to organic nitrogen; positive regulation of programmed cell death; response to hyperoxia; positive regulation of protein kinase activity; Ras protein signal transduction; cellular response to extracellular stimulus; regulation of protein import into nucleus, translocation; innate immune response; negative regulation of phosphorylation; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; negative regulation of cell growth; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle